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Quantitative Localization Microscopy: Effects of Photophysics and Labeling Stoichiometry
Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average nu...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439177/ https://www.ncbi.nlm.nih.gov/pubmed/25992915 http://dx.doi.org/10.1371/journal.pone.0127989 |