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Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection

The aim of this study was to determine the dynamic changes of dendritic cell (DC) pheno-types and T cell in response to Echinococcus multilocularis (Em) infection in BALB/c mice. Mice comprised the control and Em-infected group. At day 0, 2, 7, 30, 60, 90 and 120 after infection, the size of larval...

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Autores principales: Wei, Xiao-li, Xu, Qi, Rexiti, Fu-Lati, Zhu, Ming, Lin, Ren-yong, Wen, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Society of Experimental and Clinical Immunology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439983/
https://www.ncbi.nlm.nih.gov/pubmed/26155094
http://dx.doi.org/10.5114/ceji.2014.42117
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author Wei, Xiao-li
Xu, Qi
Rexiti, Fu-Lati
Zhu, Ming
Lin, Ren-yong
Wen, Hao
author_facet Wei, Xiao-li
Xu, Qi
Rexiti, Fu-Lati
Zhu, Ming
Lin, Ren-yong
Wen, Hao
author_sort Wei, Xiao-li
collection PubMed
description The aim of this study was to determine the dynamic changes of dendritic cell (DC) pheno-types and T cell in response to Echinococcus multilocularis (Em) infection in BALB/c mice. Mice comprised the control and Em-infected group. At day 0, 2, 7, 30, 60, 90 and 120 after infection, the size of larval cysts, the phenotype of DC and Th in splenocytes and the expression of CD40, CD86, TLR2 and TLR4, on DCs sulfur were examined. The results show that after 60 days’ infection, larval cysts grow on the surface of liver, and they become larger over time. Compared with the control mice, MHC I and MHC II expressions on DC were significantly increased at day 7 (p < 0.05). At the same time, CD40, CD86, TLR2 and TLR4 increased rapidly, but after that they decreased gradually. At day 120, those markers were lower than in the control group. The ratio of CD4/CD8 was normal during 90 days of infection, while at day 120, a decline in CD4 T cell and increase in CD8 were foundleading to the inversion of the CD4/CD8 ratio. Our findings suggest that within the 120 days of Em infection, the major function of DC is to present antigens. Immune response is provided predominantly by Th1 cells, inducing host immune response against Em. However, after 120 days, DC matured and the function was suppressed. Furthermore, inversion of the CD4/CD8 ratio is beneficial to the growth of Em, thus favoring its immune evasion.
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spelling pubmed-44399832015-07-07 Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection Wei, Xiao-li Xu, Qi Rexiti, Fu-Lati Zhu, Ming Lin, Ren-yong Wen, Hao Cent Eur J Immunol Original Article The aim of this study was to determine the dynamic changes of dendritic cell (DC) pheno-types and T cell in response to Echinococcus multilocularis (Em) infection in BALB/c mice. Mice comprised the control and Em-infected group. At day 0, 2, 7, 30, 60, 90 and 120 after infection, the size of larval cysts, the phenotype of DC and Th in splenocytes and the expression of CD40, CD86, TLR2 and TLR4, on DCs sulfur were examined. The results show that after 60 days’ infection, larval cysts grow on the surface of liver, and they become larger over time. Compared with the control mice, MHC I and MHC II expressions on DC were significantly increased at day 7 (p < 0.05). At the same time, CD40, CD86, TLR2 and TLR4 increased rapidly, but after that they decreased gradually. At day 120, those markers were lower than in the control group. The ratio of CD4/CD8 was normal during 90 days of infection, while at day 120, a decline in CD4 T cell and increase in CD8 were foundleading to the inversion of the CD4/CD8 ratio. Our findings suggest that within the 120 days of Em infection, the major function of DC is to present antigens. Immune response is provided predominantly by Th1 cells, inducing host immune response against Em. However, after 120 days, DC matured and the function was suppressed. Furthermore, inversion of the CD4/CD8 ratio is beneficial to the growth of Em, thus favoring its immune evasion. Polish Society of Experimental and Clinical Immunology 2014-04-17 2014 /pmc/articles/PMC4439983/ /pubmed/26155094 http://dx.doi.org/10.5114/ceji.2014.42117 Text en Copyright © Central European Journal of Immunology 2014 http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Wei, Xiao-li
Xu, Qi
Rexiti, Fu-Lati
Zhu, Ming
Lin, Ren-yong
Wen, Hao
Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection
title Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection
title_full Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection
title_fullStr Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection
title_full_unstemmed Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection
title_short Dynamic changes of DC and T cell subsets in mice during Echinococcus multilocularis infection
title_sort dynamic changes of dc and t cell subsets in mice during echinococcus multilocularis infection
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439983/
https://www.ncbi.nlm.nih.gov/pubmed/26155094
http://dx.doi.org/10.5114/ceji.2014.42117
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