Cargando…

Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression

The objective of this study was to determine the stimulating effect of the Biolex-Beta HP (β-1,3/1,6-D-glucan) dietary supplement on selected parameters of specific and non-specific humoral and cellular immunity in rats immunosuppressed with cyclophosphamide. The experimental material comprised 40 W...

Descripción completa

Detalles Bibliográficos
Autor principal: Wójcik, Roman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Society of Experimental and Clinical Immunology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439987/
https://www.ncbi.nlm.nih.gov/pubmed/26155100
http://dx.doi.org/10.5114/ceji.2014.42125
_version_ 1782372583716421632
author Wójcik, Roman
author_facet Wójcik, Roman
author_sort Wójcik, Roman
collection PubMed
description The objective of this study was to determine the stimulating effect of the Biolex-Beta HP (β-1,3/1,6-D-glucan) dietary supplement on selected parameters of specific and non-specific humoral and cellular immunity in rats immunosuppressed with cyclophosphamide. The experimental material comprised 40 Wistar rats, divided into two equal groups: control and experimental. In the course of 3 successive days, the rats from the experimental group were administered cyclophosphamide intramuscularly at a rate of 50 mg/kg BW per day. On the 8(th) day of the experiment, 10 control and 10 experimental rats were sacrificed, and total protein and γ-globulin levels, lysozyme and ceruloplasmin activity were determined in the blood serum. The proliferative response of blood lymphocytes after stimulation with lipopolysaccharide or concanavalin A, respiratory burst activity and the potential killing activity of phagocytes were determined in whole heparinised blood. Starting on the 8(th) day of the experiment, the feed of the remaining rats from the experimental and control groups was supplemented for 14 consecutive days with Biolex-Beta HP at a rate of 50 mg/kg BW per day. On day 22, arterial blood samples were collected and immune parameters were determined. The results indicate that β-1,3/1,6-D-glucan has a positive effect on the analysed parameters of non-specific cellular and humoral immunity after cyclophosphamide-induced suppression. Nevertheless, the observed effect only marked a return to the norm, as most of the analysed parameters were merely restored to their initial levels, with the exception of lysozyme activity, which considerably exceeded the level noted before immunosuppression.
format Online
Article
Text
id pubmed-4439987
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Polish Society of Experimental and Clinical Immunology
record_format MEDLINE/PubMed
spelling pubmed-44399872015-07-07 Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression Wójcik, Roman Cent Eur J Immunol Original Article The objective of this study was to determine the stimulating effect of the Biolex-Beta HP (β-1,3/1,6-D-glucan) dietary supplement on selected parameters of specific and non-specific humoral and cellular immunity in rats immunosuppressed with cyclophosphamide. The experimental material comprised 40 Wistar rats, divided into two equal groups: control and experimental. In the course of 3 successive days, the rats from the experimental group were administered cyclophosphamide intramuscularly at a rate of 50 mg/kg BW per day. On the 8(th) day of the experiment, 10 control and 10 experimental rats were sacrificed, and total protein and γ-globulin levels, lysozyme and ceruloplasmin activity were determined in the blood serum. The proliferative response of blood lymphocytes after stimulation with lipopolysaccharide or concanavalin A, respiratory burst activity and the potential killing activity of phagocytes were determined in whole heparinised blood. Starting on the 8(th) day of the experiment, the feed of the remaining rats from the experimental and control groups was supplemented for 14 consecutive days with Biolex-Beta HP at a rate of 50 mg/kg BW per day. On day 22, arterial blood samples were collected and immune parameters were determined. The results indicate that β-1,3/1,6-D-glucan has a positive effect on the analysed parameters of non-specific cellular and humoral immunity after cyclophosphamide-induced suppression. Nevertheless, the observed effect only marked a return to the norm, as most of the analysed parameters were merely restored to their initial levels, with the exception of lysozyme activity, which considerably exceeded the level noted before immunosuppression. Polish Society of Experimental and Clinical Immunology 2014-04-17 2014 /pmc/articles/PMC4439987/ /pubmed/26155100 http://dx.doi.org/10.5114/ceji.2014.42125 Text en Copyright © Central European Journal of Immunology 2014 http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Wójcik, Roman
Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression
title Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression
title_full Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression
title_fullStr Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression
title_full_unstemmed Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression
title_short Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression
title_sort reactivity of the immunological system of rats stimulated with biolex-beta hp after cyclophosphamide immunosuppression
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4439987/
https://www.ncbi.nlm.nih.gov/pubmed/26155100
http://dx.doi.org/10.5114/ceji.2014.42125
work_keys_str_mv AT wojcikroman reactivityoftheimmunologicalsystemofratsstimulatedwithbiolexbetahpaftercyclophosphamideimmunosuppression