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Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro

Sufentanil is a new kind of opioid analgesic and acts on μ opioid receptor. In this study, we aim to investigate the effects of sufentanil on gastric cancer cell line SGC-7901, after being exposed to different concentrations of sufentanil. Gastric cancer cells were exposed to sufentanil for a predet...

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Autores principales: Wu, Weidong, Wei, Ningxian, Jiang, Chun-nan, Cui, Suyang, Yuan, Jintao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Society of Experimental and Clinical Immunology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440010/
https://www.ncbi.nlm.nih.gov/pubmed/26155139
http://dx.doi.org/10.5114/ceji.2014.45939
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author Wu, Weidong
Wei, Ningxian
Jiang, Chun-nan
Cui, Suyang
Yuan, Jintao
author_facet Wu, Weidong
Wei, Ningxian
Jiang, Chun-nan
Cui, Suyang
Yuan, Jintao
author_sort Wu, Weidong
collection PubMed
description Sufentanil is a new kind of opioid analgesic and acts on μ opioid receptor. In this study, we aim to investigate the effects of sufentanil on gastric cancer cell line SGC-7901, after being exposed to different concentrations of sufentanil. Gastric cancer cells were exposed to sufentanil for a predetermined time at concentrations of 0, 0.5, 5, 50 and 500 nmol/l, respectively. Cell viability at different time points after exposure to sufentanil was tested by CCK-8 assay. FDA-PI staining was used to observe membrane integrity of gastric cancer SGC7901 cells. The apoptosis of gastric cancer cells was analyzed by Annexin V-FITC/PI Flow Cytometry and the changes of the cell cycle was determined by a detection kit. As a result, cell viability decreased in a dose- and time-dependent manner. Furthermore, with the concentration of sufentanil increased, the proportion of dead and apoptotic SGC-7901 cells increased, and more cells were arrested in G2/M phase. In a word, sufentanil can inhibit the cell viability and induce the apoptosis of gastric cancer SGC-7901 cells in vitro.
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spelling pubmed-44400102015-07-07 Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro Wu, Weidong Wei, Ningxian Jiang, Chun-nan Cui, Suyang Yuan, Jintao Cent Eur J Immunol Original Article Sufentanil is a new kind of opioid analgesic and acts on μ opioid receptor. In this study, we aim to investigate the effects of sufentanil on gastric cancer cell line SGC-7901, after being exposed to different concentrations of sufentanil. Gastric cancer cells were exposed to sufentanil for a predetermined time at concentrations of 0, 0.5, 5, 50 and 500 nmol/l, respectively. Cell viability at different time points after exposure to sufentanil was tested by CCK-8 assay. FDA-PI staining was used to observe membrane integrity of gastric cancer SGC7901 cells. The apoptosis of gastric cancer cells was analyzed by Annexin V-FITC/PI Flow Cytometry and the changes of the cell cycle was determined by a detection kit. As a result, cell viability decreased in a dose- and time-dependent manner. Furthermore, with the concentration of sufentanil increased, the proportion of dead and apoptotic SGC-7901 cells increased, and more cells were arrested in G2/M phase. In a word, sufentanil can inhibit the cell viability and induce the apoptosis of gastric cancer SGC-7901 cells in vitro. Polish Society of Experimental and Clinical Immunology 2014-10-14 2014 /pmc/articles/PMC4440010/ /pubmed/26155139 http://dx.doi.org/10.5114/ceji.2014.45939 Text en Copyright © Central European Journal of Immunology 2014 http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Wu, Weidong
Wei, Ningxian
Jiang, Chun-nan
Cui, Suyang
Yuan, Jintao
Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro
title Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro
title_full Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro
title_fullStr Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro
title_full_unstemmed Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro
title_short Effects of sufentanil on human gastric cancer cell line SGC-7901 in vitro
title_sort effects of sufentanil on human gastric cancer cell line sgc-7901 in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440010/
https://www.ncbi.nlm.nih.gov/pubmed/26155139
http://dx.doi.org/10.5114/ceji.2014.45939
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