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Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2

BACKGROUND: Interleukin-2 (IL-2) is a lymphocyte-activating and growth-promoting factor, and has been widely studied on T-cells and NK-cells. However, the interaction of polymorphonuclear neutrophils (PMNs) with IL-2 is poorly studied and thus, this study aimed at defining IL-2 participation in the...

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Autores principales: Abdel-Salam, Bahaa K.A., Ebaid, Hossam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Society of Experimental and Clinical Immunology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440019/
https://www.ncbi.nlm.nih.gov/pubmed/26155126
http://dx.doi.org/10.5114/ceji.2014.43725
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author Abdel-Salam, Bahaa K.A.
Ebaid, Hossam
author_facet Abdel-Salam, Bahaa K.A.
Ebaid, Hossam
author_sort Abdel-Salam, Bahaa K.A.
collection PubMed
description BACKGROUND: Interleukin-2 (IL-2) is a lymphocyte-activating and growth-promoting factor, and has been widely studied on T-cells and NK-cells. However, the interaction of polymorphonuclear neutrophils (PMNs) with IL-2 is poorly studied and thus, this study aimed at defining IL-2 participation in the expression of CD11b and CD18 on PMNs. MATERIAL AND METHODS: PMNs were isolated from heparinized whole blood of healthy donors. Purified cells were incubated with IL-2 (10 ng/ml) for 24 hours at 37°C in a humidified incubator with 5% CO(2). After 24 hours’ incubation, surface molecules (CD11b and CD18) were measured by flow cytometry. RESULTS: Interestingly, the antibodies of IL-2Rβ chain (CD122-FITC) were found in all observed cells. The induction of CD11b mean fluorescence intensity (MFI) in highly purified PMNs stimulated with IL-2 was clearly increased recording 43% in comparison to the freshly isolated PMNs and the un-stimulated PMNs which were found to be 23% and 28% of CD11b, respectively. Furthermore, flow cytometry analysis demonstrated that the highly purified PMNs exposed to IL-2 showed an increase in CD18 MFI, recording 47% with respect to that of the freshly isolated PMNs and PMNs cultured with the medium alone which showed a small amount of 38% and 27%, respectively. CONCLUSIONS: Results demonstrated that CD11b and CD18 had been acquired on the surface of the IL-2-in vitro-activated PMNs. These findings indicated that IL-2 may play a crucial role in PMNs migration.
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spelling pubmed-44400192015-07-07 Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2 Abdel-Salam, Bahaa K.A. Ebaid, Hossam Cent Eur J Immunol Original Article BACKGROUND: Interleukin-2 (IL-2) is a lymphocyte-activating and growth-promoting factor, and has been widely studied on T-cells and NK-cells. However, the interaction of polymorphonuclear neutrophils (PMNs) with IL-2 is poorly studied and thus, this study aimed at defining IL-2 participation in the expression of CD11b and CD18 on PMNs. MATERIAL AND METHODS: PMNs were isolated from heparinized whole blood of healthy donors. Purified cells were incubated with IL-2 (10 ng/ml) for 24 hours at 37°C in a humidified incubator with 5% CO(2). After 24 hours’ incubation, surface molecules (CD11b and CD18) were measured by flow cytometry. RESULTS: Interestingly, the antibodies of IL-2Rβ chain (CD122-FITC) were found in all observed cells. The induction of CD11b mean fluorescence intensity (MFI) in highly purified PMNs stimulated with IL-2 was clearly increased recording 43% in comparison to the freshly isolated PMNs and the un-stimulated PMNs which were found to be 23% and 28% of CD11b, respectively. Furthermore, flow cytometry analysis demonstrated that the highly purified PMNs exposed to IL-2 showed an increase in CD18 MFI, recording 47% with respect to that of the freshly isolated PMNs and PMNs cultured with the medium alone which showed a small amount of 38% and 27%, respectively. CONCLUSIONS: Results demonstrated that CD11b and CD18 had been acquired on the surface of the IL-2-in vitro-activated PMNs. These findings indicated that IL-2 may play a crucial role in PMNs migration. Polish Society of Experimental and Clinical Immunology 2014-06-27 2014 /pmc/articles/PMC4440019/ /pubmed/26155126 http://dx.doi.org/10.5114/ceji.2014.43725 Text en Copyright © Central European Journal of Immunology 2014 http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Abdel-Salam, Bahaa K.A.
Ebaid, Hossam
Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2
title Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2
title_full Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2
title_fullStr Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2
title_full_unstemmed Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2
title_short Expression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2
title_sort expression of cd11b and cd18 on polymorphonuclear neutrophils stimulated with interleukin-2
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440019/
https://www.ncbi.nlm.nih.gov/pubmed/26155126
http://dx.doi.org/10.5114/ceji.2014.43725
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