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A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain
The time elapsed between successful cell infection and the start of virus production is called the eclipse phase. Its duration is specific to each virus strain and, along with an effective virus production rate, plays a key role in infection kinetics. How the eclipse phase varies amongst cells infec...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440524/ https://www.ncbi.nlm.nih.gov/pubmed/25996439 http://dx.doi.org/10.1038/srep10371 |
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author | Kakizoe, Yusuke Nakaoka, Shinji Beauchemin, Catherine A. A. Morita, Satoru Mori, Hiromi Igarashi, Tatsuhiko Aihara, Kazuyuki Miura, Tomoyuki Iwami, Shingo |
author_facet | Kakizoe, Yusuke Nakaoka, Shinji Beauchemin, Catherine A. A. Morita, Satoru Mori, Hiromi Igarashi, Tatsuhiko Aihara, Kazuyuki Miura, Tomoyuki Iwami, Shingo |
author_sort | Kakizoe, Yusuke |
collection | PubMed |
description | The time elapsed between successful cell infection and the start of virus production is called the eclipse phase. Its duration is specific to each virus strain and, along with an effective virus production rate, plays a key role in infection kinetics. How the eclipse phase varies amongst cells infected with the same virus strain and therefore how best to mathematically represent its duration is not clear. Most mathematical models either neglect this phase or assume it is exponentially distributed, such that at least some if not all cells can produce virus immediately upon infection. Biologically, this is unrealistic (one must allow for the translation, transcription, export, etc. to take place), but could be appropriate if the duration of the eclipse phase is negligible on the time-scale of the infection. If it is not, however, ignoring this delay affects the accuracy of the mathematical model, its parameter estimates, and predictions. Here, we introduce a new approach, consisting in a carefully designed experiment and simple analytical expressions, to determine the duration and distribution of the eclipse phase in vitro. We find that the eclipse phase of SHIV-KS661 lasts on average one day and is consistent with an Erlang distribution. |
format | Online Article Text |
id | pubmed-4440524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44405242015-05-29 A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain Kakizoe, Yusuke Nakaoka, Shinji Beauchemin, Catherine A. A. Morita, Satoru Mori, Hiromi Igarashi, Tatsuhiko Aihara, Kazuyuki Miura, Tomoyuki Iwami, Shingo Sci Rep Article The time elapsed between successful cell infection and the start of virus production is called the eclipse phase. Its duration is specific to each virus strain and, along with an effective virus production rate, plays a key role in infection kinetics. How the eclipse phase varies amongst cells infected with the same virus strain and therefore how best to mathematically represent its duration is not clear. Most mathematical models either neglect this phase or assume it is exponentially distributed, such that at least some if not all cells can produce virus immediately upon infection. Biologically, this is unrealistic (one must allow for the translation, transcription, export, etc. to take place), but could be appropriate if the duration of the eclipse phase is negligible on the time-scale of the infection. If it is not, however, ignoring this delay affects the accuracy of the mathematical model, its parameter estimates, and predictions. Here, we introduce a new approach, consisting in a carefully designed experiment and simple analytical expressions, to determine the duration and distribution of the eclipse phase in vitro. We find that the eclipse phase of SHIV-KS661 lasts on average one day and is consistent with an Erlang distribution. Nature Publishing Group 2015-05-21 /pmc/articles/PMC4440524/ /pubmed/25996439 http://dx.doi.org/10.1038/srep10371 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kakizoe, Yusuke Nakaoka, Shinji Beauchemin, Catherine A. A. Morita, Satoru Mori, Hiromi Igarashi, Tatsuhiko Aihara, Kazuyuki Miura, Tomoyuki Iwami, Shingo A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain |
title | A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain |
title_full | A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain |
title_fullStr | A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain |
title_full_unstemmed | A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain |
title_short | A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain |
title_sort | method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic shiv strain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440524/ https://www.ncbi.nlm.nih.gov/pubmed/25996439 http://dx.doi.org/10.1038/srep10371 |
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