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Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes

BACKGROUND: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the...

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Autores principales: Paquette, Cynthia C. H., Phanse, Yashdeep, Perry, Jillian L., Sanchez-Vargas, Irma, Airs, Paul M., Dunphy, Brendan M., Xu, Jing, Carlson, Jonathan O., Luft, J. Christopher, DeSimone, Joseph M., Bartholomay, Lyric C., Beaty, Barry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440717/
https://www.ncbi.nlm.nih.gov/pubmed/25996505
http://dx.doi.org/10.1371/journal.pntd.0003745
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author Paquette, Cynthia C. H.
Phanse, Yashdeep
Perry, Jillian L.
Sanchez-Vargas, Irma
Airs, Paul M.
Dunphy, Brendan M.
Xu, Jing
Carlson, Jonathan O.
Luft, J. Christopher
DeSimone, Joseph M.
Bartholomay, Lyric C.
Beaty, Barry J.
author_facet Paquette, Cynthia C. H.
Phanse, Yashdeep
Perry, Jillian L.
Sanchez-Vargas, Irma
Airs, Paul M.
Dunphy, Brendan M.
Xu, Jing
Carlson, Jonathan O.
Luft, J. Christopher
DeSimone, Joseph M.
Bartholomay, Lyric C.
Beaty, Barry J.
author_sort Paquette, Cynthia C. H.
collection PubMed
description BACKGROUND: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes. METHODOLOGY/PRINCIPAL FINDINGS: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract. CONCLUSIONS/SIGNIFICANCE: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.
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spelling pubmed-44407172015-05-29 Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes Paquette, Cynthia C. H. Phanse, Yashdeep Perry, Jillian L. Sanchez-Vargas, Irma Airs, Paul M. Dunphy, Brendan M. Xu, Jing Carlson, Jonathan O. Luft, J. Christopher DeSimone, Joseph M. Bartholomay, Lyric C. Beaty, Barry J. PLoS Negl Trop Dis Research Article BACKGROUND: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes. METHODOLOGY/PRINCIPAL FINDINGS: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract. CONCLUSIONS/SIGNIFICANCE: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control. Public Library of Science 2015-05-21 /pmc/articles/PMC4440717/ /pubmed/25996505 http://dx.doi.org/10.1371/journal.pntd.0003745 Text en © 2015 Paquette et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Paquette, Cynthia C. H.
Phanse, Yashdeep
Perry, Jillian L.
Sanchez-Vargas, Irma
Airs, Paul M.
Dunphy, Brendan M.
Xu, Jing
Carlson, Jonathan O.
Luft, J. Christopher
DeSimone, Joseph M.
Bartholomay, Lyric C.
Beaty, Barry J.
Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes
title Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes
title_full Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes
title_fullStr Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes
title_full_unstemmed Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes
title_short Biodistribution and Trafficking of Hydrogel Nanoparticles in Adult Mosquitoes
title_sort biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440717/
https://www.ncbi.nlm.nih.gov/pubmed/25996505
http://dx.doi.org/10.1371/journal.pntd.0003745
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