Deconstructing the Polymerase Chain Reaction: Understanding and Correcting Bias Associated with Primer Degeneracies and Primer-Template Mismatches

The polymerase chain reaction (PCR) is sensitive to mismatches between primer and template, and mismatches can lead to inefficient amplification of targeted regions of DNA template. In PCRs in which a degenerate primer pool is employed, each primer can behave differently. Therefore, inefficiencies d...

Descripción completa

Detalles Bibliográficos
Autores principales: Green, Stefan J., Venkatramanan, Raghavee, Naqib, Ankur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440812/
https://www.ncbi.nlm.nih.gov/pubmed/25996930
http://dx.doi.org/10.1371/journal.pone.0128122

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440812/
https://www.ncbi.nlm.nih.gov/pubmed/25996930
http://dx.doi.org/10.1371/journal.pone.0128122

Ejemplares similares