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Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats

Hydrogen sulfide (H(2)S) plays a crucial role in the regulation of blood pressure and oxidative stress. In the present study, we tested the hypothesis that H(2)S exerts its cardiovascular effects by reducing oxidative stress via inhibition of NADPH oxidase activity in the rostral ventrolateral medul...

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Autores principales: Yu, Haiyun, Xu, Haiyan, Liu, Xiaoni, Zhang, Nana, He, Anqi, Yu, Jerry, Lu, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442288/
https://www.ncbi.nlm.nih.gov/pubmed/26078823
http://dx.doi.org/10.1155/2015/927686
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author Yu, Haiyun
Xu, Haiyan
Liu, Xiaoni
Zhang, Nana
He, Anqi
Yu, Jerry
Lu, Ning
author_facet Yu, Haiyun
Xu, Haiyan
Liu, Xiaoni
Zhang, Nana
He, Anqi
Yu, Jerry
Lu, Ning
author_sort Yu, Haiyun
collection PubMed
description Hydrogen sulfide (H(2)S) plays a crucial role in the regulation of blood pressure and oxidative stress. In the present study, we tested the hypothesis that H(2)S exerts its cardiovascular effects by reducing oxidative stress via inhibition of NADPH oxidase activity in the rostral ventrolateral medulla (RVLM). We examined cell distributions of cystathionine-β-synthase (CBS) and effects of H(2)S on reactive oxygen species (ROS) and mean arterial blood pressure (MAP) in spontaneously hypertensive rats (SHRs). We found that CBS was expressed in neurons of the RVLM, and the expression was lower in SHRs than in Wistar-Kyoto rats. Microinjection of NaHS (H(2)S donor), S-adenosyl-l-methionine (SAM, a CBS agonist), or Apocynin (NADPH oxidase inhibitor) into the RVLM reduced the ROS level, NADPH oxidase activity, and MAP, whereas microinjection of hydroxylamine hydrochloride (HA, a CBS inhibitor) increased MAP. Furthermore, intracerebroventricular infusion of NaHS inhibited phosphorylation of p47(phox), a key step of NADPH oxidase activation. Since decreasing ROS level in the RVLM reduces MAP and heart rate and increasing H(2)S reduces ROS production, we conclude that H(2)S exerts an antihypertensive effect via suppressing ROS production. H(2)S, as an antioxidant, may be a potential target for cardiovascular diseases.
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spelling pubmed-44422882015-06-15 Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats Yu, Haiyun Xu, Haiyan Liu, Xiaoni Zhang, Nana He, Anqi Yu, Jerry Lu, Ning Oxid Med Cell Longev Research Article Hydrogen sulfide (H(2)S) plays a crucial role in the regulation of blood pressure and oxidative stress. In the present study, we tested the hypothesis that H(2)S exerts its cardiovascular effects by reducing oxidative stress via inhibition of NADPH oxidase activity in the rostral ventrolateral medulla (RVLM). We examined cell distributions of cystathionine-β-synthase (CBS) and effects of H(2)S on reactive oxygen species (ROS) and mean arterial blood pressure (MAP) in spontaneously hypertensive rats (SHRs). We found that CBS was expressed in neurons of the RVLM, and the expression was lower in SHRs than in Wistar-Kyoto rats. Microinjection of NaHS (H(2)S donor), S-adenosyl-l-methionine (SAM, a CBS agonist), or Apocynin (NADPH oxidase inhibitor) into the RVLM reduced the ROS level, NADPH oxidase activity, and MAP, whereas microinjection of hydroxylamine hydrochloride (HA, a CBS inhibitor) increased MAP. Furthermore, intracerebroventricular infusion of NaHS inhibited phosphorylation of p47(phox), a key step of NADPH oxidase activation. Since decreasing ROS level in the RVLM reduces MAP and heart rate and increasing H(2)S reduces ROS production, we conclude that H(2)S exerts an antihypertensive effect via suppressing ROS production. H(2)S, as an antioxidant, may be a potential target for cardiovascular diseases. Hindawi Publishing Corporation 2015 2015-05-11 /pmc/articles/PMC4442288/ /pubmed/26078823 http://dx.doi.org/10.1155/2015/927686 Text en Copyright © 2015 Haiyun Yu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Haiyun
Xu, Haiyan
Liu, Xiaoni
Zhang, Nana
He, Anqi
Yu, Jerry
Lu, Ning
Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats
title Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats
title_full Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats
title_fullStr Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats
title_full_unstemmed Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats
title_short Superoxide Mediates Depressive Effects Induced by Hydrogen Sulfide in Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats
title_sort superoxide mediates depressive effects induced by hydrogen sulfide in rostral ventrolateral medulla of spontaneously hypertensive rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442288/
https://www.ncbi.nlm.nih.gov/pubmed/26078823
http://dx.doi.org/10.1155/2015/927686
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