Cardiac H(2)S Generation Is Reduced in Ageing Diabetic Mice

Aims. To examine whether hydrogen sulfide (H(2)S) generation changed in ageing diabetic mouse hearts. Results. Compared to mice that were fed tap water only, mice that were fed 30% fructose solution for 15 months exhibited typical characteristics of a severe diabetic phenotype with cardiac hypertrophy, fibrosis, and dysfunction. H(2)S levels in plasma, heart tissues, and urine were significantly reduced in these mice as compared to those in controls. The expression of the H(2)S-generating enzymes, cystathionine γ-lyase and 3-mercaptopyruvate sulfurtransferase, was significantly decreased in the hearts of fructose-fed mice, whereas cystathionine-β-synthase levels were significantly increased. Conclusion. Our results suggest that this ageing diabetic mouse model developed diabetic cardiomyopathy and that H(2)S levels were reduced in the diabetic heart due to alterations in three H(2)S-producing enzymes, which may be involved in the pathogenesis of diabetic cardiomyopathy.