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Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1
Muscle nuclei are exposed to variable cytoplasmic strain produced by muscle contraction and relaxation, but their morphology remains stable. Still, the mechanism responsible for maintaining myonuclear architecture, and its importance, is currently elusive. Herein, we uncovered a unique myonuclear sc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442817/ https://www.ncbi.nlm.nih.gov/pubmed/26008743 http://dx.doi.org/10.1083/jcb.201408098 |
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author | Wang, Shuoshuo Reuveny, Adriana Volk, Talila |
author_facet | Wang, Shuoshuo Reuveny, Adriana Volk, Talila |
author_sort | Wang, Shuoshuo |
collection | PubMed |
description | Muscle nuclei are exposed to variable cytoplasmic strain produced by muscle contraction and relaxation, but their morphology remains stable. Still, the mechanism responsible for maintaining myonuclear architecture, and its importance, is currently elusive. Herein, we uncovered a unique myonuclear scaffold in Drosophila melanogaster larval muscles, exhibiting both elastic features contributed by the stretching capacity of MSP300 (nesprin) and rigidity provided by a perinuclear network of microtubules stabilized by Shot (spectraplakin) and EB1. Together, they form a flexible perinuclear shield that protects myonuclei from intrinsic or extrinsic forces. The loss of this scaffold resulted in significantly aberrant nuclear morphology and subsequently reduced levels of essential nuclear factors such as lamin A/C, lamin B, and HP1. Overall, we propose a novel mechanism for maintaining myonuclear morphology and reveal its critical link to correct levels of nuclear factors in differentiated muscle fibers. These findings may shed light on the underlying mechanism of various muscular dystrophies. |
format | Online Article Text |
id | pubmed-4442817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44428172015-11-25 Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1 Wang, Shuoshuo Reuveny, Adriana Volk, Talila J Cell Biol Research Articles Muscle nuclei are exposed to variable cytoplasmic strain produced by muscle contraction and relaxation, but their morphology remains stable. Still, the mechanism responsible for maintaining myonuclear architecture, and its importance, is currently elusive. Herein, we uncovered a unique myonuclear scaffold in Drosophila melanogaster larval muscles, exhibiting both elastic features contributed by the stretching capacity of MSP300 (nesprin) and rigidity provided by a perinuclear network of microtubules stabilized by Shot (spectraplakin) and EB1. Together, they form a flexible perinuclear shield that protects myonuclei from intrinsic or extrinsic forces. The loss of this scaffold resulted in significantly aberrant nuclear morphology and subsequently reduced levels of essential nuclear factors such as lamin A/C, lamin B, and HP1. Overall, we propose a novel mechanism for maintaining myonuclear morphology and reveal its critical link to correct levels of nuclear factors in differentiated muscle fibers. These findings may shed light on the underlying mechanism of various muscular dystrophies. The Rockefeller University Press 2015-05-25 /pmc/articles/PMC4442817/ /pubmed/26008743 http://dx.doi.org/10.1083/jcb.201408098 Text en © 2015 Wang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Wang, Shuoshuo Reuveny, Adriana Volk, Talila Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1 |
title | Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1 |
title_full | Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1 |
title_fullStr | Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1 |
title_full_unstemmed | Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1 |
title_short | Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1 |
title_sort | nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and eb1 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442817/ https://www.ncbi.nlm.nih.gov/pubmed/26008743 http://dx.doi.org/10.1083/jcb.201408098 |
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