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Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment
PURPOSE: To investigate the expression profile of intravitreous cytokines, chemokines, and growth factors in patients with rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) in comparison with patients with only rhegmatogenous retinal detachment (RRD). METHODS: Twenty RRD...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443581/ https://www.ncbi.nlm.nih.gov/pubmed/26015767 |
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author | Dai, Ying Wu, Zhifeng Sheng, Huiming Zhang, Zhengwei Yu, Mengxi Zhang, Qing |
author_facet | Dai, Ying Wu, Zhifeng Sheng, Huiming Zhang, Zhengwei Yu, Mengxi Zhang, Qing |
author_sort | Dai, Ying |
collection | PubMed |
description | PURPOSE: To investigate the expression profile of intravitreous cytokines, chemokines, and growth factors in patients with rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) in comparison with patients with only rhegmatogenous retinal detachment (RRD). METHODS: Twenty RRDCD patients and 30 RRD patients were included in this case-control study. A multiplex bead-based immunoassay was performed to determine the expression of a wide range of 29 inflammatory mediators in undiluted vitreous from the patients. Data were analyzed using the Mann–Whitney U-test for nonparametric values and multivariate logistic regression analysis. RESULTS: Compared with the patients with RRD, intravitreous inflammatory mediators, including migration inhibitor factor (MIF), interleukin-6 (IL-6), CCL4, CCL11, CCL17, CCL19, CCL22, CXCL9, CXCL8, soluble inter-cellular adhesion molecule 1 (sICAM-1), transforming growth factor β3 (TGF-β3), and platelet-derived growth factor AA (PDGF-AA), were upregulated in patients with RRDCD. After calibrating the factors duration of detachment, preoperative proliferative vitreoretinopathy grade, and presence or absence of macular hole, the PDGF-AA concentrations were not significantly different according to the multivariate logistic regression analysis. MIF and sICAM-1 markers were significantly different between the two groups and represented a forward stepwise logistic regression trend. CONCLUSIONS: This is the first report to use multiplex bead analysis to investigate inflammatory mediators related to RRDCD. We proposed that the upregulated expression of these mediators may be involved in the inflammation process of RRDCD and that regulation of their expression may be potentially therapeutic by altering local inflammation. |
format | Online Article Text |
id | pubmed-4443581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-44435812015-05-26 Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment Dai, Ying Wu, Zhifeng Sheng, Huiming Zhang, Zhengwei Yu, Mengxi Zhang, Qing Mol Vis Research Article PURPOSE: To investigate the expression profile of intravitreous cytokines, chemokines, and growth factors in patients with rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) in comparison with patients with only rhegmatogenous retinal detachment (RRD). METHODS: Twenty RRDCD patients and 30 RRD patients were included in this case-control study. A multiplex bead-based immunoassay was performed to determine the expression of a wide range of 29 inflammatory mediators in undiluted vitreous from the patients. Data were analyzed using the Mann–Whitney U-test for nonparametric values and multivariate logistic regression analysis. RESULTS: Compared with the patients with RRD, intravitreous inflammatory mediators, including migration inhibitor factor (MIF), interleukin-6 (IL-6), CCL4, CCL11, CCL17, CCL19, CCL22, CXCL9, CXCL8, soluble inter-cellular adhesion molecule 1 (sICAM-1), transforming growth factor β3 (TGF-β3), and platelet-derived growth factor AA (PDGF-AA), were upregulated in patients with RRDCD. After calibrating the factors duration of detachment, preoperative proliferative vitreoretinopathy grade, and presence or absence of macular hole, the PDGF-AA concentrations were not significantly different according to the multivariate logistic regression analysis. MIF and sICAM-1 markers were significantly different between the two groups and represented a forward stepwise logistic regression trend. CONCLUSIONS: This is the first report to use multiplex bead analysis to investigate inflammatory mediators related to RRDCD. We proposed that the upregulated expression of these mediators may be involved in the inflammation process of RRDCD and that regulation of their expression may be potentially therapeutic by altering local inflammation. Molecular Vision 2015-04-10 /pmc/articles/PMC4443581/ /pubmed/26015767 Text en Copyright © 2015 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Dai, Ying Wu, Zhifeng Sheng, Huiming Zhang, Zhengwei Yu, Mengxi Zhang, Qing Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment |
title | Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment |
title_full | Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment |
title_fullStr | Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment |
title_full_unstemmed | Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment |
title_short | Identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment |
title_sort | identification of inflammatory mediators in patients with rhegmatogenous retinal detachment associated with choroidal detachment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443581/ https://www.ncbi.nlm.nih.gov/pubmed/26015767 |
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