Is mouse embryonic stem cell technology obsolete?

Injection of recombinant Cas9 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and knock-ins using the CRISPR system. These technologies may soon displace genetic modification using embryonic stem cells.

Detalles Bibliográficos
Autor principal: Skarnes, William C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research Highlight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443626/
https://www.ncbi.nlm.nih.gov/pubmed/26013980
http://dx.doi.org/10.1186/s13059-015-0673-6
Descripción
Sumario:Injection of recombinant Cas9 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and knock-ins using the CRISPR system. These technologies may soon displace genetic modification using embryonic stem cells.

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