Cargando…

A common assembly module in injectisome and flagellar type III secretion sorting platforms

Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While bo...

Descripción completa

Detalles Bibliográficos
Autores principales: Notti, Ryan Q., Bhattacharya, Shibani, Lilic, Mirjana, Stebbins, C. Erec
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443714/
https://www.ncbi.nlm.nih.gov/pubmed/25994170
http://dx.doi.org/10.1038/ncomms8125
_version_ 1782373039714861056
author Notti, Ryan Q.
Bhattacharya, Shibani
Lilic, Mirjana
Stebbins, C. Erec
author_facet Notti, Ryan Q.
Bhattacharya, Shibani
Lilic, Mirjana
Stebbins, C. Erec
author_sort Notti, Ryan Q.
collection PubMed
description Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.
format Online
Article
Text
id pubmed-4443714
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Pub. Group
record_format MEDLINE/PubMed
spelling pubmed-44437142015-06-18 A common assembly module in injectisome and flagellar type III secretion sorting platforms Notti, Ryan Q. Bhattacharya, Shibani Lilic, Mirjana Stebbins, C. Erec Nat Commun Article Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design. Nature Pub. Group 2015-05-21 /pmc/articles/PMC4443714/ /pubmed/25994170 http://dx.doi.org/10.1038/ncomms8125 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Notti, Ryan Q.
Bhattacharya, Shibani
Lilic, Mirjana
Stebbins, C. Erec
A common assembly module in injectisome and flagellar type III secretion sorting platforms
title A common assembly module in injectisome and flagellar type III secretion sorting platforms
title_full A common assembly module in injectisome and flagellar type III secretion sorting platforms
title_fullStr A common assembly module in injectisome and flagellar type III secretion sorting platforms
title_full_unstemmed A common assembly module in injectisome and flagellar type III secretion sorting platforms
title_short A common assembly module in injectisome and flagellar type III secretion sorting platforms
title_sort common assembly module in injectisome and flagellar type iii secretion sorting platforms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443714/
https://www.ncbi.nlm.nih.gov/pubmed/25994170
http://dx.doi.org/10.1038/ncomms8125
work_keys_str_mv AT nottiryanq acommonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms
AT bhattacharyashibani acommonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms
AT lilicmirjana acommonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms
AT stebbinscerec acommonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms
AT nottiryanq commonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms
AT bhattacharyashibani commonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms
AT lilicmirjana commonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms
AT stebbinscerec commonassemblymoduleininjectisomeandflagellartypeiiisecretionsortingplatforms