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Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference
Biological processes are highly dynamic but the current representation of molecular networks is static and largely qualitative. To investigate the dynamic property of genetic networks, a novel quantitative high-throughput method based on RNA interference and capable of calculating the relevance of e...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443778/ https://www.ncbi.nlm.nih.gov/pubmed/19059334 http://dx.doi.org/10.1016/j.ygeno.2008.11.006 |
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author | Fortunato, Angelo |
author_facet | Fortunato, Angelo |
author_sort | Fortunato, Angelo |
collection | PubMed |
description | Biological processes are highly dynamic but the current representation of molecular networks is static and largely qualitative. To investigate the dynamic property of genetic networks, a novel quantitative high-throughput method based on RNA interference and capable of calculating the relevance of each interaction, was developed. With this approach, it will be possible to identify not only the components of a network, but also to investigate quantitatively how network and biological processes react to perturbations. As a first application of this method, the genetic interactions of a weak loss-of-function mutation in the gene efl-1/E2F with all the genes of chromosome III were investigated during embryonic development of Caenorhabditis elegans. Fifteen synthetic genetic interactions of efl-1/E2F with the genes of chromosome III were detected, measured and ranked by statistical relevance. |
format | Online Article Text |
id | pubmed-4443778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44437782015-05-29 Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference Fortunato, Angelo Genomics Method Biological processes are highly dynamic but the current representation of molecular networks is static and largely qualitative. To investigate the dynamic property of genetic networks, a novel quantitative high-throughput method based on RNA interference and capable of calculating the relevance of each interaction, was developed. With this approach, it will be possible to identify not only the components of a network, but also to investigate quantitatively how network and biological processes react to perturbations. As a first application of this method, the genetic interactions of a weak loss-of-function mutation in the gene efl-1/E2F with all the genes of chromosome III were investigated during embryonic development of Caenorhabditis elegans. Fifteen synthetic genetic interactions of efl-1/E2F with the genes of chromosome III were detected, measured and ranked by statistical relevance. Academic Press 2009-04 /pmc/articles/PMC4443778/ /pubmed/19059334 http://dx.doi.org/10.1016/j.ygeno.2008.11.006 Text en © 2008 Elsevier Inc. All rights reserved. |
spellingShingle | Method Fortunato, Angelo Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference |
title | Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference |
title_full | Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference |
title_fullStr | Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference |
title_full_unstemmed | Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference |
title_short | Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference |
title_sort | quantitative high-throughput analysis of synthetic genetic interactions in caenorhabditis elegans by rna interference |
topic | Method |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443778/ https://www.ncbi.nlm.nih.gov/pubmed/19059334 http://dx.doi.org/10.1016/j.ygeno.2008.11.006 |
work_keys_str_mv | AT fortunatoangelo quantitativehighthroughputanalysisofsyntheticgeneticinteractionsincaenorhabditiselegansbyrnainterference |