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Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells

It is well established that metastasis through the circulatory system is primarily caused by circulating tumor cells (CTCs). In this preliminary effort, we report an approach to eliminate circulating tumor cells from the blood stream by flowing the blood though an extracorporeal tube and applying ph...

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Detalles Bibliográficos
Autores principales: Kim, Gwangseong, Gaitas, Angelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444246/
https://www.ncbi.nlm.nih.gov/pubmed/26011055
http://dx.doi.org/10.1371/journal.pone.0127219
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author Kim, Gwangseong
Gaitas, Angelo
author_facet Kim, Gwangseong
Gaitas, Angelo
author_sort Kim, Gwangseong
collection PubMed
description It is well established that metastasis through the circulatory system is primarily caused by circulating tumor cells (CTCs). In this preliminary effort, we report an approach to eliminate circulating tumor cells from the blood stream by flowing the blood though an extracorporeal tube and applying photodynamic therapy (PDT). Chlorin e6 (Ce6), a photosensitizer, was conjugated to CD44 antibody in order to target PC-3, a prostate cancer cell line. PC-3 cells were successfully stained by the Ce6-CD44 antibody conjugate. PDT was performed on whole blood spiked with stained PC-3 cells. As the blood circulated through a thin transparent medical tube, it was exposed to light of 660 nm wavelength generated by an LED array. An exposure of two minutes was sufficient to achieve selective cancer cell necrosis. In comparison, to PDT of cells growing inside a tissue culture, the PDT on thin tube exhibited significantly enhanced efficiency in cell killing, by minimizing light attenuation by blood. It suggests a new extracorporeal methodology of PDT for treating CTCs as well as other hematological pathogens.
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spelling pubmed-44442462015-06-16 Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells Kim, Gwangseong Gaitas, Angelo PLoS One Research Article It is well established that metastasis through the circulatory system is primarily caused by circulating tumor cells (CTCs). In this preliminary effort, we report an approach to eliminate circulating tumor cells from the blood stream by flowing the blood though an extracorporeal tube and applying photodynamic therapy (PDT). Chlorin e6 (Ce6), a photosensitizer, was conjugated to CD44 antibody in order to target PC-3, a prostate cancer cell line. PC-3 cells were successfully stained by the Ce6-CD44 antibody conjugate. PDT was performed on whole blood spiked with stained PC-3 cells. As the blood circulated through a thin transparent medical tube, it was exposed to light of 660 nm wavelength generated by an LED array. An exposure of two minutes was sufficient to achieve selective cancer cell necrosis. In comparison, to PDT of cells growing inside a tissue culture, the PDT on thin tube exhibited significantly enhanced efficiency in cell killing, by minimizing light attenuation by blood. It suggests a new extracorporeal methodology of PDT for treating CTCs as well as other hematological pathogens. Public Library of Science 2015-05-26 /pmc/articles/PMC4444246/ /pubmed/26011055 http://dx.doi.org/10.1371/journal.pone.0127219 Text en © 2015 Kim, Gaitas http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kim, Gwangseong
Gaitas, Angelo
Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells
title Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells
title_full Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells
title_fullStr Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells
title_full_unstemmed Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells
title_short Extracorporeal Photo-Immunotherapy for Circulating Tumor Cells
title_sort extracorporeal photo-immunotherapy for circulating tumor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444246/
https://www.ncbi.nlm.nih.gov/pubmed/26011055
http://dx.doi.org/10.1371/journal.pone.0127219
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