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Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification

Gorgonian corals are slowly declining due to human interaction and environmental impacts. Cryopreservation of gorgonian corals is an ex-situ method of conservation, ensuring future reproduction. The present study assessed the vitrification properties of cryoprotectant (CPT) mixtures using the cryoto...

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Autores principales: Tsai, Sujune, Yen, Wish, Chavanich, Suchana, Viyakarn, Voranop, Lin, Chiahsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444251/
https://www.ncbi.nlm.nih.gov/pubmed/26010144
http://dx.doi.org/10.1371/journal.pone.0123409
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author Tsai, Sujune
Yen, Wish
Chavanich, Suchana
Viyakarn, Voranop
Lin, Chiahsin
author_facet Tsai, Sujune
Yen, Wish
Chavanich, Suchana
Viyakarn, Voranop
Lin, Chiahsin
author_sort Tsai, Sujune
collection PubMed
description Gorgonian corals are slowly declining due to human interaction and environmental impacts. Cryopreservation of gorgonian corals is an ex-situ method of conservation, ensuring future reproduction. The present study assessed the vitrification properties of cryoprotectant (CPT) mixtures using the cryotop, cryoloop and open pulled straw (OPS) cryopereservation methods prior to experimentation on gorgonian (Junceella juncea) oocytes. Investigations of the equilibration and vitrification solutions’ (ES and VS) effect on oocytes throughout different incubation periods were conducted. The cryotop method was found to be the most successful in ensuring vitrification. The most favourable VS was composed of propylene glycol (PG), ethylene glycol (EG) and methanol with concentrations of 3.5M, 1.5M and 2M respectively. Experiments were performed using the cryotop method to cryopreserve Junceella juncea oocytes using VS2, the solution had the least impact on oocytes at 5°C rather than at 26°C. The success of the vitrification procedures was determined by adenosine triphosphate (ATP) levels in cooled-thaw oocytes and the highest viability obtained from the present study was 76.6 ± 6.2%. This study provides information regarding gorgonian corals’ tolerance and viability throughout vitrification to further advance the vitrification protocol on whip corals.
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spelling pubmed-44442512015-06-16 Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification Tsai, Sujune Yen, Wish Chavanich, Suchana Viyakarn, Voranop Lin, Chiahsin PLoS One Research Article Gorgonian corals are slowly declining due to human interaction and environmental impacts. Cryopreservation of gorgonian corals is an ex-situ method of conservation, ensuring future reproduction. The present study assessed the vitrification properties of cryoprotectant (CPT) mixtures using the cryotop, cryoloop and open pulled straw (OPS) cryopereservation methods prior to experimentation on gorgonian (Junceella juncea) oocytes. Investigations of the equilibration and vitrification solutions’ (ES and VS) effect on oocytes throughout different incubation periods were conducted. The cryotop method was found to be the most successful in ensuring vitrification. The most favourable VS was composed of propylene glycol (PG), ethylene glycol (EG) and methanol with concentrations of 3.5M, 1.5M and 2M respectively. Experiments were performed using the cryotop method to cryopreserve Junceella juncea oocytes using VS2, the solution had the least impact on oocytes at 5°C rather than at 26°C. The success of the vitrification procedures was determined by adenosine triphosphate (ATP) levels in cooled-thaw oocytes and the highest viability obtained from the present study was 76.6 ± 6.2%. This study provides information regarding gorgonian corals’ tolerance and viability throughout vitrification to further advance the vitrification protocol on whip corals. Public Library of Science 2015-05-26 /pmc/articles/PMC4444251/ /pubmed/26010144 http://dx.doi.org/10.1371/journal.pone.0123409 Text en © 2015 Tsai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tsai, Sujune
Yen, Wish
Chavanich, Suchana
Viyakarn, Voranop
Lin, Chiahsin
Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification
title Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification
title_full Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification
title_fullStr Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification
title_full_unstemmed Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification
title_short Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification
title_sort development of cryopreservation techniques for gorgonian (junceella juncea) oocytes through vitrification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444251/
https://www.ncbi.nlm.nih.gov/pubmed/26010144
http://dx.doi.org/10.1371/journal.pone.0123409
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