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Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7

Increasing reports suggest that deregulated microRNAs (miRNAs) might provide novel therapeutic targets for cancers. However, the expression and function of miR-300 in osteosarcoma is still unknown. In our study, we found that the expression of miR-300 was up-regulated in osteosarcoma tissues and cel...

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Detalles Bibliográficos
Autores principales: Xue, Zhen, Zhao, Jindong, Niu, Liyuan, An, Gang, Guo, Yashan, Ni, Linying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444266/
https://www.ncbi.nlm.nih.gov/pubmed/26010572
http://dx.doi.org/10.1371/journal.pone.0127682
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author Xue, Zhen
Zhao, Jindong
Niu, Liyuan
An, Gang
Guo, Yashan
Ni, Linying
author_facet Xue, Zhen
Zhao, Jindong
Niu, Liyuan
An, Gang
Guo, Yashan
Ni, Linying
author_sort Xue, Zhen
collection PubMed
description Increasing reports suggest that deregulated microRNAs (miRNAs) might provide novel therapeutic targets for cancers. However, the expression and function of miR-300 in osteosarcoma is still unknown. In our study, we found that the expression of miR-300 was up-regulated in osteosarcoma tissues and cells compared with paired adjacent non-tumor bone tissues and osteoblastic cells using RT-qPCR. The enforced expression of miR-300 could promote cell proliferation, invasion and epithelial-mesenchymal transition (EMT). Moreover, we identified that bromodomain-containing protein 7 (BRD7), a new tumor suppressor gene, was a direct target of miR-300. Ectopic expression of BRD7 could significantly inhibit miR-300-promoted proliferation, invasion and EMT. Therefore, our results identify an important role for miR-300 in osteosarcoma through regulating BRD7 expression.
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spelling pubmed-44442662015-06-16 Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7 Xue, Zhen Zhao, Jindong Niu, Liyuan An, Gang Guo, Yashan Ni, Linying PLoS One Research Article Increasing reports suggest that deregulated microRNAs (miRNAs) might provide novel therapeutic targets for cancers. However, the expression and function of miR-300 in osteosarcoma is still unknown. In our study, we found that the expression of miR-300 was up-regulated in osteosarcoma tissues and cells compared with paired adjacent non-tumor bone tissues and osteoblastic cells using RT-qPCR. The enforced expression of miR-300 could promote cell proliferation, invasion and epithelial-mesenchymal transition (EMT). Moreover, we identified that bromodomain-containing protein 7 (BRD7), a new tumor suppressor gene, was a direct target of miR-300. Ectopic expression of BRD7 could significantly inhibit miR-300-promoted proliferation, invasion and EMT. Therefore, our results identify an important role for miR-300 in osteosarcoma through regulating BRD7 expression. Public Library of Science 2015-05-26 /pmc/articles/PMC4444266/ /pubmed/26010572 http://dx.doi.org/10.1371/journal.pone.0127682 Text en © 2015 Xue et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xue, Zhen
Zhao, Jindong
Niu, Liyuan
An, Gang
Guo, Yashan
Ni, Linying
Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7
title Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7
title_full Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7
title_fullStr Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7
title_full_unstemmed Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7
title_short Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7
title_sort up-regulation of mir-300 promotes proliferation and invasion of osteosarcoma by targeting brd7
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444266/
https://www.ncbi.nlm.nih.gov/pubmed/26010572
http://dx.doi.org/10.1371/journal.pone.0127682
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