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Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes

The aim of this work was to assess OchratoxinA (OTA) production of different Aspergillus carbonarius isolates, evaluate their growth profile through different growth measurements, and reveal any underlying correlation between them. Ten different isolates of A. carbonarius isolated from Greek vineyar...

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Autores principales: Lappa, Iliada K., Kizis, Dimosthenis, Natskoulis, Pantelis I., Panagou, Efstathios Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444842/
https://www.ncbi.nlm.nih.gov/pubmed/26074896
http://dx.doi.org/10.3389/fmicb.2015.00502
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author Lappa, Iliada K.
Kizis, Dimosthenis
Natskoulis, Pantelis I.
Panagou, Efstathios Z.
author_facet Lappa, Iliada K.
Kizis, Dimosthenis
Natskoulis, Pantelis I.
Panagou, Efstathios Z.
author_sort Lappa, Iliada K.
collection PubMed
description The aim of this work was to assess OchratoxinA (OTA) production of different Aspergillus carbonarius isolates, evaluate their growth profile through different growth measurements, and reveal any underlying correlation between them. Ten different isolates of A. carbonarius isolated from Greek vineyards located in different geographical regions were examined in vitro for their OTA production potential after an incubation period of up to 11 days. All fungal isolates grew on a synthetic grape juice medium (SGM) similar to grape composition at optimum conditions of temperature and water activity (25°C and 0.98 a(w)). Samples for OTA determination were removed at 3, 5, 7, 9, and 11 days of growth and analyzed by HPLC. Based on OTA measurements the isolates were characterized by diverse OTA production ranging from 50 to 2000 ppb at day 11. The different fungal growth responses (colony diameter, colony area, biomass, biomass dry weight, and colony density) have been measured and correlated with toxin production by means of principal components analysis (PCA), confirming satisfactory correlation and explained over 99% of data variability. Leudeking-Piret model was also used to study OTA production with time, revealing a mixed-growth associated trend and pointing a fail-safe model with slightly better prediction through colony area. This approach contributes to the assessment of correlation between mycotoxin production and different methods of fungal growth determination in relation to time.
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spelling pubmed-44448422015-06-12 Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes Lappa, Iliada K. Kizis, Dimosthenis Natskoulis, Pantelis I. Panagou, Efstathios Z. Front Microbiol Microbiology The aim of this work was to assess OchratoxinA (OTA) production of different Aspergillus carbonarius isolates, evaluate their growth profile through different growth measurements, and reveal any underlying correlation between them. Ten different isolates of A. carbonarius isolated from Greek vineyards located in different geographical regions were examined in vitro for their OTA production potential after an incubation period of up to 11 days. All fungal isolates grew on a synthetic grape juice medium (SGM) similar to grape composition at optimum conditions of temperature and water activity (25°C and 0.98 a(w)). Samples for OTA determination were removed at 3, 5, 7, 9, and 11 days of growth and analyzed by HPLC. Based on OTA measurements the isolates were characterized by diverse OTA production ranging from 50 to 2000 ppb at day 11. The different fungal growth responses (colony diameter, colony area, biomass, biomass dry weight, and colony density) have been measured and correlated with toxin production by means of principal components analysis (PCA), confirming satisfactory correlation and explained over 99% of data variability. Leudeking-Piret model was also used to study OTA production with time, revealing a mixed-growth associated trend and pointing a fail-safe model with slightly better prediction through colony area. This approach contributes to the assessment of correlation between mycotoxin production and different methods of fungal growth determination in relation to time. Frontiers Media S.A. 2015-05-27 /pmc/articles/PMC4444842/ /pubmed/26074896 http://dx.doi.org/10.3389/fmicb.2015.00502 Text en Copyright © 2015 Lappa, Kizis, Natskoulis and Panagou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lappa, Iliada K.
Kizis, Dimosthenis
Natskoulis, Pantelis I.
Panagou, Efstathios Z.
Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes
title Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes
title_full Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes
title_fullStr Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes
title_full_unstemmed Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes
title_short Comparative study of growth responses and screening of inter-specific OTA production kinetics by A. carbonarius isolated from grapes
title_sort comparative study of growth responses and screening of inter-specific ota production kinetics by a. carbonarius isolated from grapes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444842/
https://www.ncbi.nlm.nih.gov/pubmed/26074896
http://dx.doi.org/10.3389/fmicb.2015.00502
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