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Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo

Toxoplasma gondii is a highly prevalent intracellular protozoan parasite that causes severe disease in congenitally infected or immunocompromised hosts. T. gondii is capable of invading immune cells and it has been suggested that the parasite harnesses the migratory pathways of these cells to spread...

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Autores principales: Ueno, Norikiyo, Lodoen, Melissa B, Hickey, Graeme L, Robey, Ellen A, Coombes, Janine L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446200/
https://www.ncbi.nlm.nih.gov/pubmed/25533287
http://dx.doi.org/10.1038/icb.2014.106
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author Ueno, Norikiyo
Lodoen, Melissa B
Hickey, Graeme L
Robey, Ellen A
Coombes, Janine L
author_facet Ueno, Norikiyo
Lodoen, Melissa B
Hickey, Graeme L
Robey, Ellen A
Coombes, Janine L
author_sort Ueno, Norikiyo
collection PubMed
description Toxoplasma gondii is a highly prevalent intracellular protozoan parasite that causes severe disease in congenitally infected or immunocompromised hosts. T. gondii is capable of invading immune cells and it has been suggested that the parasite harnesses the migratory pathways of these cells to spread through the body. Although in vitro evidence suggests that the parasite further enhances its spread by inducing a hypermotility phenotype in parasitized immune cells, in vivo evidence for this phenomenon is scarce. Here we use a physiologically relevant oral model of T. gondii infection, in conjunction with two-photon laser scanning microscopy, to address this issue. We found that a small proportion of natural killer (NK) cells in mesenteric lymph nodes contained parasites. Compared with uninfected ‘bystander' NK cells, these infected NK cells showed faster, more directed and more persistent migratory behavior. Consistent with this, infected NK cells showed impaired spreading and clustering of the integrin, LFA-1, when exposed to plated ligands. Our results provide the first evidence for a hypermigratory phenotype in T. gondii-infected NK cells in vivo, providing an anatomical context for understanding how the parasite manipulates immune cell motility to spread through the host.
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spelling pubmed-44462002015-06-09 Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo Ueno, Norikiyo Lodoen, Melissa B Hickey, Graeme L Robey, Ellen A Coombes, Janine L Immunol Cell Biol Short Communication Toxoplasma gondii is a highly prevalent intracellular protozoan parasite that causes severe disease in congenitally infected or immunocompromised hosts. T. gondii is capable of invading immune cells and it has been suggested that the parasite harnesses the migratory pathways of these cells to spread through the body. Although in vitro evidence suggests that the parasite further enhances its spread by inducing a hypermotility phenotype in parasitized immune cells, in vivo evidence for this phenomenon is scarce. Here we use a physiologically relevant oral model of T. gondii infection, in conjunction with two-photon laser scanning microscopy, to address this issue. We found that a small proportion of natural killer (NK) cells in mesenteric lymph nodes contained parasites. Compared with uninfected ‘bystander' NK cells, these infected NK cells showed faster, more directed and more persistent migratory behavior. Consistent with this, infected NK cells showed impaired spreading and clustering of the integrin, LFA-1, when exposed to plated ligands. Our results provide the first evidence for a hypermigratory phenotype in T. gondii-infected NK cells in vivo, providing an anatomical context for understanding how the parasite manipulates immune cell motility to spread through the host. Nature Publishing Group 2015-05 2014-12-23 /pmc/articles/PMC4446200/ /pubmed/25533287 http://dx.doi.org/10.1038/icb.2014.106 Text en Copyright © 2015 Australasian Society for Immunology Inc. http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Short Communication
Ueno, Norikiyo
Lodoen, Melissa B
Hickey, Graeme L
Robey, Ellen A
Coombes, Janine L
Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo
title Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo
title_full Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo
title_fullStr Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo
title_full_unstemmed Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo
title_short Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo
title_sort toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446200/
https://www.ncbi.nlm.nih.gov/pubmed/25533287
http://dx.doi.org/10.1038/icb.2014.106
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