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High-Resolution and Specific Detection of Bacteria on Complex Surfaces Using Nanoparticle Probes and Electron Microscopy

The study of the interaction of bacteria with surfaces requires the detection of specific bacterial groups with high spatial resolution. Here, we describe a method to rapidly and efficiently add nanogold particles to oligonucleotide probes, which target bacterial ribosomal RNA. These nanogold-labele...

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Detalles Bibliográficos
Autores principales: Ye, Jun, Nielsen, Shaun, Joseph, Stephen, Thomas, Torsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446341/
https://www.ncbi.nlm.nih.gov/pubmed/26018431
http://dx.doi.org/10.1371/journal.pone.0126404