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Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA

Functions of prokaryotic Argonautes (pAgo) have long remained elusive. Recently, Argonautes of the bacteria Rhodobacter sphaeroides and Thermus thermophilus were demonstrated to be involved in host defense. The Argonaute of the archaeon Pyrococcus furiosus (PfAgo) belongs to a different branch in th...

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Autores principales: Swarts, Daan C., Hegge, Jorrit W., Hinojo, Ismael, Shiimori, Masami, Ellis, Michael A., Dumrongkulraksa, Justin, Terns, Rebecca M., Terns, Michael P., van der Oost, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446448/
https://www.ncbi.nlm.nih.gov/pubmed/25925567
http://dx.doi.org/10.1093/nar/gkv415
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author Swarts, Daan C.
Hegge, Jorrit W.
Hinojo, Ismael
Shiimori, Masami
Ellis, Michael A.
Dumrongkulraksa, Justin
Terns, Rebecca M.
Terns, Michael P.
van der Oost, John
author_facet Swarts, Daan C.
Hegge, Jorrit W.
Hinojo, Ismael
Shiimori, Masami
Ellis, Michael A.
Dumrongkulraksa, Justin
Terns, Rebecca M.
Terns, Michael P.
van der Oost, John
author_sort Swarts, Daan C.
collection PubMed
description Functions of prokaryotic Argonautes (pAgo) have long remained elusive. Recently, Argonautes of the bacteria Rhodobacter sphaeroides and Thermus thermophilus were demonstrated to be involved in host defense. The Argonaute of the archaeon Pyrococcus furiosus (PfAgo) belongs to a different branch in the phylogenetic tree, which is most closely related to that of RNA interference-mediating eukaryotic Argonautes. Here we describe a functional and mechanistic characterization of PfAgo. Like the bacterial counterparts, archaeal PfAgo contributes to host defense by interfering with the uptake of plasmid DNA. PfAgo utilizes small 5′-phosphorylated DNA guides to cleave both single stranded and double stranded DNA targets, and does not utilize RNA as guide or target. Thus, with respect to function and specificity, the archaeal PfAgo resembles bacterial Argonautes much more than eukaryotic Argonautes. These findings demonstrate that the role of Argonautes is conserved through the bacterial and archaeal domains of life and suggests that eukaryotic Argonautes are derived from DNA-guided DNA-interfering host defense systems.
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spelling pubmed-44464482015-06-15 Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA Swarts, Daan C. Hegge, Jorrit W. Hinojo, Ismael Shiimori, Masami Ellis, Michael A. Dumrongkulraksa, Justin Terns, Rebecca M. Terns, Michael P. van der Oost, John Nucleic Acids Res Nucleic Acid Enzymes Functions of prokaryotic Argonautes (pAgo) have long remained elusive. Recently, Argonautes of the bacteria Rhodobacter sphaeroides and Thermus thermophilus were demonstrated to be involved in host defense. The Argonaute of the archaeon Pyrococcus furiosus (PfAgo) belongs to a different branch in the phylogenetic tree, which is most closely related to that of RNA interference-mediating eukaryotic Argonautes. Here we describe a functional and mechanistic characterization of PfAgo. Like the bacterial counterparts, archaeal PfAgo contributes to host defense by interfering with the uptake of plasmid DNA. PfAgo utilizes small 5′-phosphorylated DNA guides to cleave both single stranded and double stranded DNA targets, and does not utilize RNA as guide or target. Thus, with respect to function and specificity, the archaeal PfAgo resembles bacterial Argonautes much more than eukaryotic Argonautes. These findings demonstrate that the role of Argonautes is conserved through the bacterial and archaeal domains of life and suggests that eukaryotic Argonautes are derived from DNA-guided DNA-interfering host defense systems. Oxford University Press 2015-05-26 2015-04-29 /pmc/articles/PMC4446448/ /pubmed/25925567 http://dx.doi.org/10.1093/nar/gkv415 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Nucleic Acid Enzymes
Swarts, Daan C.
Hegge, Jorrit W.
Hinojo, Ismael
Shiimori, Masami
Ellis, Michael A.
Dumrongkulraksa, Justin
Terns, Rebecca M.
Terns, Michael P.
van der Oost, John
Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
title Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
title_full Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
title_fullStr Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
title_full_unstemmed Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
title_short Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
title_sort argonaute of the archaeon pyrococcus furiosus is a dna-guided nuclease that targets cognate dna
topic Nucleic Acid Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446448/
https://www.ncbi.nlm.nih.gov/pubmed/25925567
http://dx.doi.org/10.1093/nar/gkv415
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