Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli

BACKGROUND: In general, fusion of recombinant genes to strong inducible promoters allowing intracellular expression in Bacillus subtilis is a two-step process. The ligation products are transformed into Escherichia coli, followed by identification of the correct plasmid, and this plasmid is subseque...

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Detalles Bibliográficos
Autores principales: Phan, Trang Thi Phuong, Tran, Linh Thuoc, Schumann, Wolfgang, Nguyen, Hoang Duc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446860/
https://www.ncbi.nlm.nih.gov/pubmed/25990516
http://dx.doi.org/10.1186/s12934-015-0255-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446860/
https://www.ncbi.nlm.nih.gov/pubmed/25990516
http://dx.doi.org/10.1186/s12934-015-0255-z

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