Improved amplification efficiency on stool samples by addition of spermidine and its use for non-invasive detection of colorectal cancer

BACKGROUND: Using quantitative methylation-specific PCR (QM-MSP) is a promising method for colorectal cancer (CRC) diagnosis from stool samples. Difficulty in eliminating PCR inhibitors of this body fluid has been extensively reported. Here, spermidine is presented as PCR facilitator for the detecti...

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Detalles Bibliográficos
Autores principales: Roperch, Jean-Pierre, Benzekri, Karim, Mansour, Hicham, Incitti, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446959/
https://www.ncbi.nlm.nih.gov/pubmed/26022272
http://dx.doi.org/10.1186/s12896-015-0148-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446959/
https://www.ncbi.nlm.nih.gov/pubmed/26022272
http://dx.doi.org/10.1186/s12896-015-0148-6

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