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Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells

BACKGROUND: N-myc down-regulated gene 2 (NDRG2) is a tumor suppressor involved in cell proliferation and differentiation. The aim of this study was to determine the uterine expression pattern of this gene during early pregnancy in mice. METHODS: Uterine NDRG2 mRNA and protein expression levels were...

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Autores principales: Gu, Yan, Zhang, Xuan, Yang, Qian, Wang, Jian-mei, He, Ya-ping, Sun, Zhao-gui, Zhang, Hui-qin, Wang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447025/
https://www.ncbi.nlm.nih.gov/pubmed/26013399
http://dx.doi.org/10.1186/s12958-015-0047-7
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author Gu, Yan
Zhang, Xuan
Yang, Qian
Wang, Jian-mei
He, Ya-ping
Sun, Zhao-gui
Zhang, Hui-qin
Wang, Jian
author_facet Gu, Yan
Zhang, Xuan
Yang, Qian
Wang, Jian-mei
He, Ya-ping
Sun, Zhao-gui
Zhang, Hui-qin
Wang, Jian
author_sort Gu, Yan
collection PubMed
description BACKGROUND: N-myc down-regulated gene 2 (NDRG2) is a tumor suppressor involved in cell proliferation and differentiation. The aim of this study was to determine the uterine expression pattern of this gene during early pregnancy in mice. METHODS: Uterine NDRG2 mRNA and protein expression levels were determined by RT-PCR and Western blot analyses, respectively, during the peri-implantation period in mice. Immunohistochemical (IHC) analysis was performed to examine the spatial localization of NDRG2 expression in mouse uterine tissues. The in vitro decidualization model of mouse endometrial stromal cells (ESCs) was used to evaluate decidualization of ESCs following NDRG2 knock down by small interfering RNA (siRNA). Statistical significance was analyzed by one-way ANOVA using SPSS 19.0 software. RESULTS: Uterine NDRG2 gene expression was significantly up-regulated and was predominantly localized to the secondary decidual zone on days 5 and 8 of pregnancy in mice. Its increased expression was associated with artificial decidualization as well as the activation of delayed implantation. Furthermore, uterine NDRG2 expression was induced by estrogen and progesterone treatments. The in vitro decidualization of mouse ESCs was accompanied by up-regulation of NDRG2 expression, and knock down of its expression in these cells by siRNA inhibited the decidualization process. CONCLUSIONS: These results suggest that NDRG2 might play an important role in the process of decidualization during early pregnancy.
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spelling pubmed-44470252015-05-29 Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells Gu, Yan Zhang, Xuan Yang, Qian Wang, Jian-mei He, Ya-ping Sun, Zhao-gui Zhang, Hui-qin Wang, Jian Reprod Biol Endocrinol Research BACKGROUND: N-myc down-regulated gene 2 (NDRG2) is a tumor suppressor involved in cell proliferation and differentiation. The aim of this study was to determine the uterine expression pattern of this gene during early pregnancy in mice. METHODS: Uterine NDRG2 mRNA and protein expression levels were determined by RT-PCR and Western blot analyses, respectively, during the peri-implantation period in mice. Immunohistochemical (IHC) analysis was performed to examine the spatial localization of NDRG2 expression in mouse uterine tissues. The in vitro decidualization model of mouse endometrial stromal cells (ESCs) was used to evaluate decidualization of ESCs following NDRG2 knock down by small interfering RNA (siRNA). Statistical significance was analyzed by one-way ANOVA using SPSS 19.0 software. RESULTS: Uterine NDRG2 gene expression was significantly up-regulated and was predominantly localized to the secondary decidual zone on days 5 and 8 of pregnancy in mice. Its increased expression was associated with artificial decidualization as well as the activation of delayed implantation. Furthermore, uterine NDRG2 expression was induced by estrogen and progesterone treatments. The in vitro decidualization of mouse ESCs was accompanied by up-regulation of NDRG2 expression, and knock down of its expression in these cells by siRNA inhibited the decidualization process. CONCLUSIONS: These results suggest that NDRG2 might play an important role in the process of decidualization during early pregnancy. BioMed Central 2015-05-27 /pmc/articles/PMC4447025/ /pubmed/26013399 http://dx.doi.org/10.1186/s12958-015-0047-7 Text en © Gu et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Gu, Yan
Zhang, Xuan
Yang, Qian
Wang, Jian-mei
He, Ya-ping
Sun, Zhao-gui
Zhang, Hui-qin
Wang, Jian
Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells
title Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells
title_full Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells
title_fullStr Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells
title_full_unstemmed Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells
title_short Uterine NDRG2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells
title_sort uterine ndrg2 expression is increased at implantation sites during early pregnancy in mice, and its down-regulation inhibits decidualization of mouse endometrial stromal cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447025/
https://www.ncbi.nlm.nih.gov/pubmed/26013399
http://dx.doi.org/10.1186/s12958-015-0047-7
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