Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles

In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample...

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Detalles Bibliográficos
Autores principales: Ilovitsh, Tali, Danan, Yossef, Meir, Rinat, Meiri, Amihai, Zalevsky, Zeev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447069/
https://www.ncbi.nlm.nih.gov/pubmed/26020693
http://dx.doi.org/10.1038/srep10965
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author Ilovitsh, Tali
Danan, Yossef
Meir, Rinat
Meiri, Amihai
Zalevsky, Zeev
author_facet Ilovitsh, Tali
Danan, Yossef
Meir, Rinat
Meiri, Amihai
Zalevsky, Zeev
author_sort Ilovitsh, Tali
collection PubMed
description In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample. The final image where the GNPs are spatially separated is obtained computationally. The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.
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spelling pubmed-44470692015-06-10 Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles Ilovitsh, Tali Danan, Yossef Meir, Rinat Meiri, Amihai Zalevsky, Zeev Sci Rep Article In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample. The final image where the GNPs are spatially separated is obtained computationally. The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells. Nature Publishing Group 2015-05-28 /pmc/articles/PMC4447069/ /pubmed/26020693 http://dx.doi.org/10.1038/srep10965 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ilovitsh, Tali
Danan, Yossef
Meir, Rinat
Meiri, Amihai
Zalevsky, Zeev
Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles
title Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles
title_full Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles
title_fullStr Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles
title_full_unstemmed Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles
title_short Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles
title_sort cellular superresolved imaging of multiple markers using temporally flickering nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447069/
https://www.ncbi.nlm.nih.gov/pubmed/26020693
http://dx.doi.org/10.1038/srep10965
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