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Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals

The Lubin underground mine, is one of three mining divisions in the Lubin-Glogow Copper District in Lower Silesia province (Poland). It is the source of polymetallic ore that is rich in copper, silver and several heavy metals. Black shale is also significantly enriched in fossil organic matter in th...

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Autores principales: Dziewit, Lukasz, Pyzik, Adam, Szuplewska, Magdalena, Matlakowska, Renata, Mielnicki, Sebastian, Wibberg, Daniel, Schlüter, Andreas, Pühler, Alfred, Bartosik, Dariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447125/
https://www.ncbi.nlm.nih.gov/pubmed/26074880
http://dx.doi.org/10.3389/fmicb.2015.00152
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author Dziewit, Lukasz
Pyzik, Adam
Szuplewska, Magdalena
Matlakowska, Renata
Mielnicki, Sebastian
Wibberg, Daniel
Schlüter, Andreas
Pühler, Alfred
Bartosik, Dariusz
author_facet Dziewit, Lukasz
Pyzik, Adam
Szuplewska, Magdalena
Matlakowska, Renata
Mielnicki, Sebastian
Wibberg, Daniel
Schlüter, Andreas
Pühler, Alfred
Bartosik, Dariusz
author_sort Dziewit, Lukasz
collection PubMed
description The Lubin underground mine, is one of three mining divisions in the Lubin-Glogow Copper District in Lower Silesia province (Poland). It is the source of polymetallic ore that is rich in copper, silver and several heavy metals. Black shale is also significantly enriched in fossil organic matter in the form of long-chain hydrocarbons, polycyclic aromatic hydrocarbons, organic acids, esters, thiophenes and metalloporphyrins. Biological analyses have revealed that this environment is inhabited by extremophilic bacteria and fungi. Kupfershiefer black shale and samples of water, bottom and mineral sediments from the underground (below 600 m) Lubin mine were taken and 20 bacterial strains were isolated and characterized. All exhibited multi-resistant and hypertolerant phenotypes to heavy metals. We analyzed the plasmidome of these strains in order to evaluate the diversity and role of mobile DNA in adaptation to the harsh conditions of the mine environment. Experimental and bioinformatic analyses of 11 extrachromosomal replicons were performed. Three plasmids, including a broad-host-range replicon containing a Tn3 family transposon, carried genes conferring resistance to arsenic, cadmium, cobalt, mercury and zinc. Functional analysis revealed that the resistance modules exhibit host specificity, i.e., they may increase or decrease tolerance to toxic ions depending on the host strain. The other identified replicons showed diverse features. Among them we identified a catabolic plasmid encoding enzymes involved in the utilization of histidine and vanillate, a putative plasmid-like prophage carrying genes responsible for NAD biosynthesis, and two repABC-type plasmids containing virulence-associated genes. These findings provide an unique molecular insight into the pool of extrachromosomal replicons and highlight their role in the biology and adaptation of extremophilic bacteria inhabiting terrestrial deep subsurface.
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spelling pubmed-44471252015-06-12 Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals Dziewit, Lukasz Pyzik, Adam Szuplewska, Magdalena Matlakowska, Renata Mielnicki, Sebastian Wibberg, Daniel Schlüter, Andreas Pühler, Alfred Bartosik, Dariusz Front Microbiol Microbiology The Lubin underground mine, is one of three mining divisions in the Lubin-Glogow Copper District in Lower Silesia province (Poland). It is the source of polymetallic ore that is rich in copper, silver and several heavy metals. Black shale is also significantly enriched in fossil organic matter in the form of long-chain hydrocarbons, polycyclic aromatic hydrocarbons, organic acids, esters, thiophenes and metalloporphyrins. Biological analyses have revealed that this environment is inhabited by extremophilic bacteria and fungi. Kupfershiefer black shale and samples of water, bottom and mineral sediments from the underground (below 600 m) Lubin mine were taken and 20 bacterial strains were isolated and characterized. All exhibited multi-resistant and hypertolerant phenotypes to heavy metals. We analyzed the plasmidome of these strains in order to evaluate the diversity and role of mobile DNA in adaptation to the harsh conditions of the mine environment. Experimental and bioinformatic analyses of 11 extrachromosomal replicons were performed. Three plasmids, including a broad-host-range replicon containing a Tn3 family transposon, carried genes conferring resistance to arsenic, cadmium, cobalt, mercury and zinc. Functional analysis revealed that the resistance modules exhibit host specificity, i.e., they may increase or decrease tolerance to toxic ions depending on the host strain. The other identified replicons showed diverse features. Among them we identified a catabolic plasmid encoding enzymes involved in the utilization of histidine and vanillate, a putative plasmid-like prophage carrying genes responsible for NAD biosynthesis, and two repABC-type plasmids containing virulence-associated genes. These findings provide an unique molecular insight into the pool of extrachromosomal replicons and highlight their role in the biology and adaptation of extremophilic bacteria inhabiting terrestrial deep subsurface. Frontiers Media S.A. 2015-03-03 /pmc/articles/PMC4447125/ /pubmed/26074880 http://dx.doi.org/10.3389/fmicb.2015.00152 Text en Copyright © 2015 Dziewit, Pyzik, Szuplewska, Matlakowska, Mielnicki, Wibberg, Schlüter, Pühler and Bartosik. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dziewit, Lukasz
Pyzik, Adam
Szuplewska, Magdalena
Matlakowska, Renata
Mielnicki, Sebastian
Wibberg, Daniel
Schlüter, Andreas
Pühler, Alfred
Bartosik, Dariusz
Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals
title Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals
title_full Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals
title_fullStr Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals
title_full_unstemmed Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals
title_short Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals
title_sort diversity and role of plasmids in adaptation of bacteria inhabiting the lubin copper mine in poland, an environment rich in heavy metals
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447125/
https://www.ncbi.nlm.nih.gov/pubmed/26074880
http://dx.doi.org/10.3389/fmicb.2015.00152
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