Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages

Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB ser...

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Detalles Bibliográficos
Autores principales: Monk, Ian R., Tree, Jai J., Howden, Benjamin P., Stinear, Timothy P., Foster, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447248/
https://www.ncbi.nlm.nih.gov/pubmed/26015493
http://dx.doi.org/10.1128/mBio.00308-15
Descripción
Sumario:Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of S. aureus clonal complexes 1, 8, 30, and ST93. The IMXXB strains enable direct, high-efficiency transformation and streamlined genetic manipulation of major S. aureus lineages.

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