Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages

Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB ser...

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Autores principales: Monk, Ian R., Tree, Jai J., Howden, Benjamin P., Stinear, Timothy P., Foster, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447248/
https://www.ncbi.nlm.nih.gov/pubmed/26015493
http://dx.doi.org/10.1128/mBio.00308-15
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author Monk, Ian R.
Tree, Jai J.
Howden, Benjamin P.
Stinear, Timothy P.
Foster, Timothy J.
author_facet Monk, Ian R.
Tree, Jai J.
Howden, Benjamin P.
Stinear, Timothy P.
Foster, Timothy J.
author_sort Monk, Ian R.
collection PubMed
description Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of S. aureus clonal complexes 1, 8, 30, and ST93. The IMXXB strains enable direct, high-efficiency transformation and streamlined genetic manipulation of major S. aureus lineages.
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spelling pubmed-44472482015-05-30 Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages Monk, Ian R. Tree, Jai J. Howden, Benjamin P. Stinear, Timothy P. Foster, Timothy J. mBio Research Article Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of S. aureus clonal complexes 1, 8, 30, and ST93. The IMXXB strains enable direct, high-efficiency transformation and streamlined genetic manipulation of major S. aureus lineages. American Society of Microbiology 2015-05-26 /pmc/articles/PMC4447248/ /pubmed/26015493 http://dx.doi.org/10.1128/mBio.00308-15 Text en Copyright © 2015 Monk et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Monk, Ian R.
Tree, Jai J.
Howden, Benjamin P.
Stinear, Timothy P.
Foster, Timothy J.
Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
title Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
title_full Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
title_fullStr Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
title_full_unstemmed Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
title_short Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
title_sort complete bypass of restriction systems for major staphylococcus aureus lineages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447248/
https://www.ncbi.nlm.nih.gov/pubmed/26015493
http://dx.doi.org/10.1128/mBio.00308-15
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