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Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Extracellular vesicles (EVs) play a significant role in cell–cell communication in numerous physiological processes and pathological conditions, and offer promise as novel biomarkers and therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that c...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Co-Action Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447833/ https://www.ncbi.nlm.nih.gov/pubmed/26022510 http://dx.doi.org/10.3402/jev.v4.26883 |
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author | Li, Jinghuan Lee, Yi Johansson, Henrik J. Mäger, Imre Vader, Pieter Nordin, Joel Z. Wiklander, Oscar P. B. Lehtiö, Janne Wood, Matthew J. A. Andaloussi, Samir EL |
author_facet | Li, Jinghuan Lee, Yi Johansson, Henrik J. Mäger, Imre Vader, Pieter Nordin, Joel Z. Wiklander, Oscar P. B. Lehtiö, Janne Wood, Matthew J. A. Andaloussi, Samir EL |
author_sort | Li, Jinghuan |
collection | PubMed |
description | Extracellular vesicles (EVs) play a significant role in cell–cell communication in numerous physiological processes and pathological conditions, and offer promise as novel biomarkers and therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis and release from cells. However, little is known about how external stimuli such as cell culture conditions can affect the quantity and content of EVs. While N2a neuroblastoma cells cultured in serum-free (OptiMEM) conditions did not result in EVs with significant biophysical or size differences compared with cells cultured in serum-containing (pre-spun) conditions, the quantity of isolated EVs was greatly increased. Moreover, the expression levels of certain vesicular proteins (e.g. small GTPases, G-protein complexes, mRNA processing proteins and splicing factors), some of which were previously reported to be involved in EV biogenesis, were found to be differentially expressed in EVs under different culture conditions. These data, therefore, contribute to the understanding of how extracellular factors and intracellular molecular pathways affect the composition and release of EVs. |
format | Online Article Text |
id | pubmed-4447833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Co-Action Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-44478332015-06-15 Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles Li, Jinghuan Lee, Yi Johansson, Henrik J. Mäger, Imre Vader, Pieter Nordin, Joel Z. Wiklander, Oscar P. B. Lehtiö, Janne Wood, Matthew J. A. Andaloussi, Samir EL J Extracell Vesicles Original Research Article Extracellular vesicles (EVs) play a significant role in cell–cell communication in numerous physiological processes and pathological conditions, and offer promise as novel biomarkers and therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis and release from cells. However, little is known about how external stimuli such as cell culture conditions can affect the quantity and content of EVs. While N2a neuroblastoma cells cultured in serum-free (OptiMEM) conditions did not result in EVs with significant biophysical or size differences compared with cells cultured in serum-containing (pre-spun) conditions, the quantity of isolated EVs was greatly increased. Moreover, the expression levels of certain vesicular proteins (e.g. small GTPases, G-protein complexes, mRNA processing proteins and splicing factors), some of which were previously reported to be involved in EV biogenesis, were found to be differentially expressed in EVs under different culture conditions. These data, therefore, contribute to the understanding of how extracellular factors and intracellular molecular pathways affect the composition and release of EVs. Co-Action Publishing 2015-05-27 /pmc/articles/PMC4447833/ /pubmed/26022510 http://dx.doi.org/10.3402/jev.v4.26883 Text en © 2015 Jinghuan Li et al. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Article Li, Jinghuan Lee, Yi Johansson, Henrik J. Mäger, Imre Vader, Pieter Nordin, Joel Z. Wiklander, Oscar P. B. Lehtiö, Janne Wood, Matthew J. A. Andaloussi, Samir EL Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
title | Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
title_full | Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
title_fullStr | Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
title_full_unstemmed | Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
title_short | Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
title_sort | serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447833/ https://www.ncbi.nlm.nih.gov/pubmed/26022510 http://dx.doi.org/10.3402/jev.v4.26883 |
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