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Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography

Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Ce...

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Detalles Bibliográficos
Autores principales: Lu, Lina, Qi, Zhijun, Zhang, Jiwen, Wu, Wenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4448171/
https://www.ncbi.nlm.nih.gov/pubmed/25996604
http://dx.doi.org/10.3390/toxins7051738
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author Lu, Lina
Qi, Zhijun
Zhang, Jiwen
Wu, Wenjun
author_facet Lu, Lina
Qi, Zhijun
Zhang, Jiwen
Wu, Wenjun
author_sort Lu, Lina
collection PubMed
description Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V. Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae. The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed.
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spelling pubmed-44481712015-06-01 Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography Lu, Lina Qi, Zhijun Zhang, Jiwen Wu, Wenjun Toxins (Basel) Article Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V. Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae. The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed. MDPI 2015-05-19 /pmc/articles/PMC4448171/ /pubmed/25996604 http://dx.doi.org/10.3390/toxins7051738 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Lina
Qi, Zhijun
Zhang, Jiwen
Wu, Wenjun
Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
title Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
title_full Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
title_fullStr Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
title_full_unstemmed Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
title_short Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
title_sort separation of binding protein of celangulin v from the midgut of mythimna separata walker by affinity chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4448171/
https://www.ncbi.nlm.nih.gov/pubmed/25996604
http://dx.doi.org/10.3390/toxins7051738
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