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Validation of a locked nucleic acid based wild-type blocking PCR for the detection of EGFR exon 18/19 mutations

BACKGROUND: Treatment decisions in advanced non-small cell lung cancer rely on accurate analysis of the EGFR mutation status in small tissue samples. Sanger sequencing of PCR products is unbiased and cheap, but its detection threshold requiring 20 % infiltration by malignant cells is not optimal. Co...

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Detalles Bibliográficos
Autores principales:	Vliegen, Liesbet, Dooms, Christophe, De Kelver, Wim, Verbeken, Eric, Vansteenkiste, Johan, Vandenberghe, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4448309/ https://www.ncbi.nlm.nih.gov/pubmed/26022577 http://dx.doi.org/10.1186/s13000-015-0293-1

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