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The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster
Considerable evidence indicates that sleep is essential for learning and memory. Drosophila melanogaster has emerged as a novel model for studying sleep. We previously found a short sleeper mutant, fumin (fmn), and identified its mutation in the dopamine transporter gene. We reported similarities in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449117/ https://www.ncbi.nlm.nih.gov/pubmed/26023770 http://dx.doi.org/10.1371/journal.pone.0128101 |
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author | Tomita, Jun Ueno, Taro Mitsuyoshi, Madoka Kume, Shoen Kume, Kazuhiko |
author_facet | Tomita, Jun Ueno, Taro Mitsuyoshi, Madoka Kume, Shoen Kume, Kazuhiko |
author_sort | Tomita, Jun |
collection | PubMed |
description | Considerable evidence indicates that sleep is essential for learning and memory. Drosophila melanogaster has emerged as a novel model for studying sleep. We previously found a short sleeper mutant, fumin (fmn), and identified its mutation in the dopamine transporter gene. We reported similarities in the molecular basis of sleep and arousal regulation between mammals and Drosophila. In aversive olfactory learning tasks, fmn mutants demonstrate defective memory retention, which suggests an association between sleep and memory. In an attempt to discover additional sleep related genes in Drosophila, we carried out a microarray analysis comparing mRNA expression in heads of fmn and control flies and found that 563 genes are differentially expressed. Next, using the pan-neuronal Gal4 driver elav-Gal4 and UAS-RNA interference (RNAi) to knockdown individual genes, we performed a functional screen. We found that knockdown of the NMDA type glutamate receptor channel gene (Nmdar1) (also known as dNR1) reduced sleep. The NMDA receptor (NMDAR) plays an important role in learning and memory both in Drosophila and mammals. The application of the NMDAR antagonist, MK-801, reduced sleep in control flies, but not in fmn. These results suggest that NMDAR promotes sleep regulation in Drosophila. |
format | Online Article Text |
id | pubmed-4449117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44491172015-06-09 The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster Tomita, Jun Ueno, Taro Mitsuyoshi, Madoka Kume, Shoen Kume, Kazuhiko PLoS One Research Article Considerable evidence indicates that sleep is essential for learning and memory. Drosophila melanogaster has emerged as a novel model for studying sleep. We previously found a short sleeper mutant, fumin (fmn), and identified its mutation in the dopamine transporter gene. We reported similarities in the molecular basis of sleep and arousal regulation between mammals and Drosophila. In aversive olfactory learning tasks, fmn mutants demonstrate defective memory retention, which suggests an association between sleep and memory. In an attempt to discover additional sleep related genes in Drosophila, we carried out a microarray analysis comparing mRNA expression in heads of fmn and control flies and found that 563 genes are differentially expressed. Next, using the pan-neuronal Gal4 driver elav-Gal4 and UAS-RNA interference (RNAi) to knockdown individual genes, we performed a functional screen. We found that knockdown of the NMDA type glutamate receptor channel gene (Nmdar1) (also known as dNR1) reduced sleep. The NMDA receptor (NMDAR) plays an important role in learning and memory both in Drosophila and mammals. The application of the NMDAR antagonist, MK-801, reduced sleep in control flies, but not in fmn. These results suggest that NMDAR promotes sleep regulation in Drosophila. Public Library of Science 2015-05-29 /pmc/articles/PMC4449117/ /pubmed/26023770 http://dx.doi.org/10.1371/journal.pone.0128101 Text en © 2015 Tomita et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tomita, Jun Ueno, Taro Mitsuyoshi, Madoka Kume, Shoen Kume, Kazuhiko The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster |
title | The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster
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title_full | The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster
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title_fullStr | The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster
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title_full_unstemmed | The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster
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title_short | The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster
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title_sort | nmda receptor promotes sleep in the fruit fly, drosophila melanogaster |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449117/ https://www.ncbi.nlm.nih.gov/pubmed/26023770 http://dx.doi.org/10.1371/journal.pone.0128101 |
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