Cargando…

The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes

The traditional definition of climacteric and non-climacteric fruits has been put into question. A significant example of this paradox is the climacteric fig fruit. Surprisingly, ripening-related ethylene production increases following pre- or postharvest 1-methylcyclopropene (1-MCP) application in...

Descripción completa

Detalles Bibliográficos
Autores principales: Freiman, Zohar E., Rosianskey, Yogev, Dasmohapatra, Rajeswari, Kamara, Itzhak, Flaishman, Moshe A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449545/
https://www.ncbi.nlm.nih.gov/pubmed/25956879
http://dx.doi.org/10.1093/jxb/erv140
_version_ 1782373872744529920
author Freiman, Zohar E.
Rosianskey, Yogev
Dasmohapatra, Rajeswari
Kamara, Itzhak
Flaishman, Moshe A.
author_facet Freiman, Zohar E.
Rosianskey, Yogev
Dasmohapatra, Rajeswari
Kamara, Itzhak
Flaishman, Moshe A.
author_sort Freiman, Zohar E.
collection PubMed
description The traditional definition of climacteric and non-climacteric fruits has been put into question. A significant example of this paradox is the climacteric fig fruit. Surprisingly, ripening-related ethylene production increases following pre- or postharvest 1-methylcyclopropene (1-MCP) application in an unexpected auto-inhibitory manner. In this study, ethylene production and the expression of potential ripening-regulator, ethylene-synthesis, and signal-transduction genes are characterized in figs ripening on the tree and following preharvest 1-MCP application. Fig ripening-related gene expression was similar to that in tomato and apple during ripening on the tree, but only in the fig inflorescence–drupelet section. Because the pattern in the receptacle is different for most of the genes, the fig drupelets developed inside the syconium are proposed to function as parthenocarpic true fruit, regulating ripening processes for the whole accessory fruit. Transcription of a potential ripening regulator, FcMADS8, increased during ripening on the tree and was inhibited following 1-MCP treatment. Expression patterns of the ethylene-synthesis genes FcACS2, FcACS4, and FcACO3 could be related to the auto-inhibition reaction of ethylene production in 1-MCP-treated fruit. Along with FcMADS8 suppression, gene expression analysis revealed upregulation of FcEBF1, and downregulation of FcEIL3 and several FcERFs by 1-MCP treatment. This corresponded with the high storability of the treated fruit. One FcERF was overexpressed in the 1-MCP-treated fruit, and did not share the increasing pattern of most FcERFs in the tree-ripened fig. This demonstrates the potential of this downstream ethylene-signal-transduction component as an ethylene-synthesis regulator, responsible for the non-climacteric auto-inhibition of ethylene production in fig.
format Online
Article
Text
id pubmed-4449545
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-44495452015-06-05 The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes Freiman, Zohar E. Rosianskey, Yogev Dasmohapatra, Rajeswari Kamara, Itzhak Flaishman, Moshe A. J Exp Bot Research Paper The traditional definition of climacteric and non-climacteric fruits has been put into question. A significant example of this paradox is the climacteric fig fruit. Surprisingly, ripening-related ethylene production increases following pre- or postharvest 1-methylcyclopropene (1-MCP) application in an unexpected auto-inhibitory manner. In this study, ethylene production and the expression of potential ripening-regulator, ethylene-synthesis, and signal-transduction genes are characterized in figs ripening on the tree and following preharvest 1-MCP application. Fig ripening-related gene expression was similar to that in tomato and apple during ripening on the tree, but only in the fig inflorescence–drupelet section. Because the pattern in the receptacle is different for most of the genes, the fig drupelets developed inside the syconium are proposed to function as parthenocarpic true fruit, regulating ripening processes for the whole accessory fruit. Transcription of a potential ripening regulator, FcMADS8, increased during ripening on the tree and was inhibited following 1-MCP treatment. Expression patterns of the ethylene-synthesis genes FcACS2, FcACS4, and FcACO3 could be related to the auto-inhibition reaction of ethylene production in 1-MCP-treated fruit. Along with FcMADS8 suppression, gene expression analysis revealed upregulation of FcEBF1, and downregulation of FcEIL3 and several FcERFs by 1-MCP treatment. This corresponded with the high storability of the treated fruit. One FcERF was overexpressed in the 1-MCP-treated fruit, and did not share the increasing pattern of most FcERFs in the tree-ripened fig. This demonstrates the potential of this downstream ethylene-signal-transduction component as an ethylene-synthesis regulator, responsible for the non-climacteric auto-inhibition of ethylene production in fig. Oxford University Press 2015-06 2015-05-08 /pmc/articles/PMC4449545/ /pubmed/25956879 http://dx.doi.org/10.1093/jxb/erv140 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Freiman, Zohar E.
Rosianskey, Yogev
Dasmohapatra, Rajeswari
Kamara, Itzhak
Flaishman, Moshe A.
The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes
title The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes
title_full The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes
title_fullStr The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes
title_full_unstemmed The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes
title_short The ambiguous ripening nature of the fig (Ficus carica L.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes
title_sort ambiguous ripening nature of the fig (ficus carica l.) fruit: a gene-expression study of potential ripening regulators and ethylene-related genes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449545/
https://www.ncbi.nlm.nih.gov/pubmed/25956879
http://dx.doi.org/10.1093/jxb/erv140
work_keys_str_mv AT freimanzohare theambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT rosianskeyyogev theambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT dasmohapatrarajeswari theambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT kamaraitzhak theambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT flaishmanmoshea theambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT freimanzohare ambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT rosianskeyyogev ambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT dasmohapatrarajeswari ambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT kamaraitzhak ambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes
AT flaishmanmoshea ambiguousripeningnatureofthefigficuscaricalfruitageneexpressionstudyofpotentialripeningregulatorsandethylenerelatedgenes