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The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer

BACKGROUND: We aimed to examine the expression of miR-1307 in chemosensitive and chemoresistant epithelial ovarian cancer tissues and cell lines and to analyze the clinicopathological significance of miR-1307 in ovarian cancer. METHODS: MicroRNA microarray was used to screen differentially expressed...

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Autores principales: Zhou, Yingying, Wang, Min, Wu, Jianlei, Jie, Zhihui, Chang, Shuang, Shuang, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449560/
https://www.ncbi.nlm.nih.gov/pubmed/25887170
http://dx.doi.org/10.1186/s13048-015-0143-5
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author Zhou, Yingying
Wang, Min
Wu, Jianlei
Jie, Zhihui
Chang, Shuang
Shuang, Ting
author_facet Zhou, Yingying
Wang, Min
Wu, Jianlei
Jie, Zhihui
Chang, Shuang
Shuang, Ting
author_sort Zhou, Yingying
collection PubMed
description BACKGROUND: We aimed to examine the expression of miR-1307 in chemosensitive and chemoresistant epithelial ovarian cancer tissues and cell lines and to analyze the clinicopathological significance of miR-1307 in ovarian cancer. METHODS: MicroRNA microarray was used to screen differentially expressed microRNAs between the chemosensitive and chemoresistant epithelial ovarian cancer tissues. RT-PCR was used to validate the candidate microRNA. The potential target genes and their enriched biological pathways of microRNA were also analyzed. Dual Luciferase Reporter Gene Assay was conducted to validate the regulation of miRNA-1307 on the 3’-UTR of DAPK3. RESULTS: miRNA-1307 was up-regulated in the chemoresistant epithelial ovarian cancer tissues compared to the chemosensitive counterparts. The up-regulation of miRNA-1307 was not associated with menopause, tumor differentiation state, clinical stage, and lymph node metastasis of ovarian cancer. Gene ontology analysis of miR-1307 candidate target genes indicated that miR-1307 candidate target genes were enriched in the processes of cell proliferation and differentiation, nucleotide synthesis and metabolism, and lymphocytes activation. CONCLUSION: Our results suggest that miRNA-1307 may play a role in the development of chemoresistance in ovarian cancer. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13048-015-0143-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-44495602015-05-31 The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer Zhou, Yingying Wang, Min Wu, Jianlei Jie, Zhihui Chang, Shuang Shuang, Ting J Ovarian Res Research BACKGROUND: We aimed to examine the expression of miR-1307 in chemosensitive and chemoresistant epithelial ovarian cancer tissues and cell lines and to analyze the clinicopathological significance of miR-1307 in ovarian cancer. METHODS: MicroRNA microarray was used to screen differentially expressed microRNAs between the chemosensitive and chemoresistant epithelial ovarian cancer tissues. RT-PCR was used to validate the candidate microRNA. The potential target genes and their enriched biological pathways of microRNA were also analyzed. Dual Luciferase Reporter Gene Assay was conducted to validate the regulation of miRNA-1307 on the 3’-UTR of DAPK3. RESULTS: miRNA-1307 was up-regulated in the chemoresistant epithelial ovarian cancer tissues compared to the chemosensitive counterparts. The up-regulation of miRNA-1307 was not associated with menopause, tumor differentiation state, clinical stage, and lymph node metastasis of ovarian cancer. Gene ontology analysis of miR-1307 candidate target genes indicated that miR-1307 candidate target genes were enriched in the processes of cell proliferation and differentiation, nucleotide synthesis and metabolism, and lymphocytes activation. CONCLUSION: Our results suggest that miRNA-1307 may play a role in the development of chemoresistance in ovarian cancer. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13048-015-0143-5) contains supplementary material, which is available to authorized users. BioMed Central 2015-04-09 /pmc/articles/PMC4449560/ /pubmed/25887170 http://dx.doi.org/10.1186/s13048-015-0143-5 Text en © Zhou et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhou, Yingying
Wang, Min
Wu, Jianlei
Jie, Zhihui
Chang, Shuang
Shuang, Ting
The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer
title The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer
title_full The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer
title_fullStr The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer
title_full_unstemmed The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer
title_short The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer
title_sort clinicopathological significance of mir-1307 in chemotherapy resistant epithelial ovarian cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449560/
https://www.ncbi.nlm.nih.gov/pubmed/25887170
http://dx.doi.org/10.1186/s13048-015-0143-5
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