The effect of immunological status, in-vitro treatment and culture time on expression of eleven candidate reference genes in bovine blood mononuclear cells

BACKGROUND: Technical feasibility of RNA quantification by real time RT-PCR has led to enormous utilization of this method. However, real time PCR results need to be normalized due to the high sensitivity of the method and also to eliminate technical variation. Normalization against a reference gene...

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Detalles Bibliográficos
Autores principales: Emam, Mehdi, Thompson-Crispi, Kathleen, Mallard, Bonnie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449592/
https://www.ncbi.nlm.nih.gov/pubmed/26025301
http://dx.doi.org/10.1186/s12865-015-0099-7

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