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Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers

Aims. We describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify and compare simultaneously global methylation and hydroxymethylation in human DNA of different tissues. Materials and Methods. Blood and saliva DNA from fourteen volunteers was processed for epigenetic e...

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Autores principales: Godderis, Lode, Schouteden, Caroline, Tabish, Ali, Poels, Katrien, Hoet, Peter, Baccarelli, Andrea A., Van Landuyt, Kirsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450276/
https://www.ncbi.nlm.nih.gov/pubmed/26090450
http://dx.doi.org/10.1155/2015/845041
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author Godderis, Lode
Schouteden, Caroline
Tabish, Ali
Poels, Katrien
Hoet, Peter
Baccarelli, Andrea A.
Van Landuyt, Kirsten
author_facet Godderis, Lode
Schouteden, Caroline
Tabish, Ali
Poels, Katrien
Hoet, Peter
Baccarelli, Andrea A.
Van Landuyt, Kirsten
author_sort Godderis, Lode
collection PubMed
description Aims. We describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify and compare simultaneously global methylation and hydroxymethylation in human DNA of different tissues. Materials and Methods. Blood and saliva DNA from fourteen volunteers was processed for epigenetic endpoints using LC-MS/MS and PCR-pyrosequencing technology. Results. Global DNA methylation was significantly lower in saliva (mean 4.61% ±  0.80%), compared to blood samples (5.70% ± 0.22%). In contrast, saliva (0.036% ± 0.011%) revealed significantly higher hydroxymethylation compared to blood samples (mean 0.027% ± 0.004%). Whereas we did not find significant correlations for both epigenetic measures between the tissues, a significant association was observed between global methylation and global hydroxymethylation in saliva DNA. Neither LINE-1 nor Alu elements of blood and saliva correlated, nor were they correlated with the DNA hydroxymethylation of blood or saliva, respectively. Conclusion. Global DNA methylation and hydroxymethylation of cytosine can be quantified simultaneously by LC-MS/MS. Saliva DNA cannot be considered as a surrogate for blood DNA to study epigenetic endpoints.
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spelling pubmed-44502762015-06-18 Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers Godderis, Lode Schouteden, Caroline Tabish, Ali Poels, Katrien Hoet, Peter Baccarelli, Andrea A. Van Landuyt, Kirsten Biomed Res Int Research Article Aims. We describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify and compare simultaneously global methylation and hydroxymethylation in human DNA of different tissues. Materials and Methods. Blood and saliva DNA from fourteen volunteers was processed for epigenetic endpoints using LC-MS/MS and PCR-pyrosequencing technology. Results. Global DNA methylation was significantly lower in saliva (mean 4.61% ±  0.80%), compared to blood samples (5.70% ± 0.22%). In contrast, saliva (0.036% ± 0.011%) revealed significantly higher hydroxymethylation compared to blood samples (mean 0.027% ± 0.004%). Whereas we did not find significant correlations for both epigenetic measures between the tissues, a significant association was observed between global methylation and global hydroxymethylation in saliva DNA. Neither LINE-1 nor Alu elements of blood and saliva correlated, nor were they correlated with the DNA hydroxymethylation of blood or saliva, respectively. Conclusion. Global DNA methylation and hydroxymethylation of cytosine can be quantified simultaneously by LC-MS/MS. Saliva DNA cannot be considered as a surrogate for blood DNA to study epigenetic endpoints. Hindawi Publishing Corporation 2015 2015-05-18 /pmc/articles/PMC4450276/ /pubmed/26090450 http://dx.doi.org/10.1155/2015/845041 Text en Copyright © 2015 Lode Godderis et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Godderis, Lode
Schouteden, Caroline
Tabish, Ali
Poels, Katrien
Hoet, Peter
Baccarelli, Andrea A.
Van Landuyt, Kirsten
Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers
title Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers
title_full Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers
title_fullStr Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers
title_full_unstemmed Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers
title_short Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers
title_sort global methylation and hydroxymethylation in dna from blood and saliva in healthy volunteers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450276/
https://www.ncbi.nlm.nih.gov/pubmed/26090450
http://dx.doi.org/10.1155/2015/845041
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