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Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts

Euphorbia helioscopia L. is a traditional Chinese medicine; recently research found that its ethyl acetate extract (EAE) plays an important role on tumor cell proliferation, apoptosis, invasion, and metastasis in vitro. But the effect of EAE for tumor cells in vivo has not been reported. To explore...

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Autores principales: Cheng, Junsheng, Han, Wei, Wang, Zheyuan, Shao, Yuan, Wang, Yingzhen, Zhang, Yawu, Li, Zhongxin, Xu, Xiaodong, Zhang, Youcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450285/
https://www.ncbi.nlm.nih.gov/pubmed/26090427
http://dx.doi.org/10.1155/2015/601015
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author Cheng, Junsheng
Han, Wei
Wang, Zheyuan
Shao, Yuan
Wang, Yingzhen
Zhang, Yawu
Li, Zhongxin
Xu, Xiaodong
Zhang, Youcheng
author_facet Cheng, Junsheng
Han, Wei
Wang, Zheyuan
Shao, Yuan
Wang, Yingzhen
Zhang, Yawu
Li, Zhongxin
Xu, Xiaodong
Zhang, Youcheng
author_sort Cheng, Junsheng
collection PubMed
description Euphorbia helioscopia L. is a traditional Chinese medicine; recently research found that its ethyl acetate extract (EAE) plays an important role on tumor cell proliferation, apoptosis, invasion, and metastasis in vitro. But the effect of EAE for tumor cells in vivo has not been reported. To explore the inhibitory effect of EAE and molecular mechanism on hepatocellular carcinoma (HCC) SMMC-7721 cells in vivo, we utilized the nude mouse xenograft model of HCC. Treated with EAE (50, 100, and 200 μg/mL), the volume of xenograft was measured during the entire process of EAE treatment. In EAE treatment group, the volume of xenograft was significantly reduced compared with the control group (P < 0.05) and the protein expressions of CyclinD1, bcl-2, and MMP-9 were reduced, while those of bax, caspase-3, and nm23-H1 were increased. A significant change trend with increasing EAE concentrations has presented, compared with controls. Moreover, the ultrastructural morphology of xenografts showed significant changes, including nuclear pyknosis and chromatin condensation, We found that EAE could effectively inhibit tumor growth, induce apoptosis, and inhibit tumor invasion and metastasis in vivo; it is suggested that EAE is a potential candidate for as a new anticancer agent.
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spelling pubmed-44502852015-06-18 Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts Cheng, Junsheng Han, Wei Wang, Zheyuan Shao, Yuan Wang, Yingzhen Zhang, Yawu Li, Zhongxin Xu, Xiaodong Zhang, Youcheng Biomed Res Int Research Article Euphorbia helioscopia L. is a traditional Chinese medicine; recently research found that its ethyl acetate extract (EAE) plays an important role on tumor cell proliferation, apoptosis, invasion, and metastasis in vitro. But the effect of EAE for tumor cells in vivo has not been reported. To explore the inhibitory effect of EAE and molecular mechanism on hepatocellular carcinoma (HCC) SMMC-7721 cells in vivo, we utilized the nude mouse xenograft model of HCC. Treated with EAE (50, 100, and 200 μg/mL), the volume of xenograft was measured during the entire process of EAE treatment. In EAE treatment group, the volume of xenograft was significantly reduced compared with the control group (P < 0.05) and the protein expressions of CyclinD1, bcl-2, and MMP-9 were reduced, while those of bax, caspase-3, and nm23-H1 were increased. A significant change trend with increasing EAE concentrations has presented, compared with controls. Moreover, the ultrastructural morphology of xenografts showed significant changes, including nuclear pyknosis and chromatin condensation, We found that EAE could effectively inhibit tumor growth, induce apoptosis, and inhibit tumor invasion and metastasis in vivo; it is suggested that EAE is a potential candidate for as a new anticancer agent. Hindawi Publishing Corporation 2015 2015-05-18 /pmc/articles/PMC4450285/ /pubmed/26090427 http://dx.doi.org/10.1155/2015/601015 Text en Copyright © 2015 Junsheng Cheng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cheng, Junsheng
Han, Wei
Wang, Zheyuan
Shao, Yuan
Wang, Yingzhen
Zhang, Yawu
Li, Zhongxin
Xu, Xiaodong
Zhang, Youcheng
Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts
title Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts
title_full Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts
title_fullStr Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts
title_full_unstemmed Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts
title_short Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts
title_sort hepatocellular carcinoma growth is inhibited by euphorbia helioscopia l. extract in nude mice xenografts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450285/
https://www.ncbi.nlm.nih.gov/pubmed/26090427
http://dx.doi.org/10.1155/2015/601015
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