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Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis

The incidence of osteoporosis is high in postmenopausal women due to altered estrogen levels and continuous calcium loss that occurs with aging. Recent studies have shown that microRNAs (miRNAs) are involved in the development of osteoporosis. These miRNAs may be used as potential biomarkers to iden...

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Autores principales: Meng, Jia, Zhang, Dapeng, Pan, Nanan, Sun, Ning, Wang, Qiujun, Fan, Jingxue, Zhou, Ping, Zhu, Wenliang, Jiang, Lihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451039/
https://www.ncbi.nlm.nih.gov/pubmed/26038726
http://dx.doi.org/10.7717/peerj.971
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author Meng, Jia
Zhang, Dapeng
Pan, Nanan
Sun, Ning
Wang, Qiujun
Fan, Jingxue
Zhou, Ping
Zhu, Wenliang
Jiang, Lihong
author_facet Meng, Jia
Zhang, Dapeng
Pan, Nanan
Sun, Ning
Wang, Qiujun
Fan, Jingxue
Zhou, Ping
Zhu, Wenliang
Jiang, Lihong
author_sort Meng, Jia
collection PubMed
description The incidence of osteoporosis is high in postmenopausal women due to altered estrogen levels and continuous calcium loss that occurs with aging. Recent studies have shown that microRNAs (miRNAs) are involved in the development of osteoporosis. These miRNAs may be used as potential biomarkers to identify women at a high risk for developing the disease. In this study, whole blood samples were collected from 48 postmenopausal Chinese women with osteopenia or osteoporosis and pooled into six groups according to individual T-scores. A miRNA microarray analysis was performed on pooled blood samples to identify potential miRNA biomarkers for postmenopausal osteoporosis. Five miRNAs (miR-130b-3p, -151a-3p, -151b, -194-5p, and -590-5p) were identified in the microarray analysis. These dysregulated miRNAs were subjected to a pathway analysis investigating whether they were involved in regulating osteoporosis-related pathways. Among them, only miR-194-5p was enriched in multiple osteoporosis-related pathways. Enhanced miR-194-5p expression in women with osteoporosis was confirmed by quantitative reverse transcription–polymerase chain reaction analysis. For external validation, a significant correlation between the expression of miR-194-5p and T-scores was found in an independent patient collection comprised of 24 postmenopausal women with normal bone mineral density, 30 postmenopausal women with osteopenia, and 32 postmenopausal women with osteoporosis (p < 0.05). Taken together, the present findings suggest that miR-194-5p may be a viable miRNA biomarker for postmenopausal osteoporosis.
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spelling pubmed-44510392015-06-02 Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis Meng, Jia Zhang, Dapeng Pan, Nanan Sun, Ning Wang, Qiujun Fan, Jingxue Zhou, Ping Zhu, Wenliang Jiang, Lihong PeerJ Molecular Biology The incidence of osteoporosis is high in postmenopausal women due to altered estrogen levels and continuous calcium loss that occurs with aging. Recent studies have shown that microRNAs (miRNAs) are involved in the development of osteoporosis. These miRNAs may be used as potential biomarkers to identify women at a high risk for developing the disease. In this study, whole blood samples were collected from 48 postmenopausal Chinese women with osteopenia or osteoporosis and pooled into six groups according to individual T-scores. A miRNA microarray analysis was performed on pooled blood samples to identify potential miRNA biomarkers for postmenopausal osteoporosis. Five miRNAs (miR-130b-3p, -151a-3p, -151b, -194-5p, and -590-5p) were identified in the microarray analysis. These dysregulated miRNAs were subjected to a pathway analysis investigating whether they were involved in regulating osteoporosis-related pathways. Among them, only miR-194-5p was enriched in multiple osteoporosis-related pathways. Enhanced miR-194-5p expression in women with osteoporosis was confirmed by quantitative reverse transcription–polymerase chain reaction analysis. For external validation, a significant correlation between the expression of miR-194-5p and T-scores was found in an independent patient collection comprised of 24 postmenopausal women with normal bone mineral density, 30 postmenopausal women with osteopenia, and 32 postmenopausal women with osteoporosis (p < 0.05). Taken together, the present findings suggest that miR-194-5p may be a viable miRNA biomarker for postmenopausal osteoporosis. PeerJ Inc. 2015-05-21 /pmc/articles/PMC4451039/ /pubmed/26038726 http://dx.doi.org/10.7717/peerj.971 Text en © 2015 Meng et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Molecular Biology
Meng, Jia
Zhang, Dapeng
Pan, Nanan
Sun, Ning
Wang, Qiujun
Fan, Jingxue
Zhou, Ping
Zhu, Wenliang
Jiang, Lihong
Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis
title Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis
title_full Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis
title_fullStr Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis
title_full_unstemmed Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis
title_short Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis
title_sort identification of mir-194-5p as a potential biomarker for postmenopausal osteoporosis
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451039/
https://www.ncbi.nlm.nih.gov/pubmed/26038726
http://dx.doi.org/10.7717/peerj.971
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