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Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells
The functions of 1, 25-dihydroxyvitamin D (1, 25-(OH)(2)D(3)) in regulating adipogenesis, adipocyte differentiation and key adipogenic gene expression were studied in 3T3-L1 preadipocytes. Five concentrations (0.01, 0.1, 1, 10, 100nM) of 1, 25-(OH)(2)D(3) were studied and lipid accumulation measured...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451075/ https://www.ncbi.nlm.nih.gov/pubmed/26030589 http://dx.doi.org/10.1371/journal.pone.0126142 |
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author | Ji, Shuhan Doumit, Matthew E. Hill, Rodney A. |
author_facet | Ji, Shuhan Doumit, Matthew E. Hill, Rodney A. |
author_sort | Ji, Shuhan |
collection | PubMed |
description | The functions of 1, 25-dihydroxyvitamin D (1, 25-(OH)(2)D(3)) in regulating adipogenesis, adipocyte differentiation and key adipogenic gene expression were studied in 3T3-L1 preadipocytes. Five concentrations (0.01, 0.1, 1, 10, 100nM) of 1, 25-(OH)(2)D(3) were studied and lipid accumulation measured by Oil Red O staining and expression of adipogenic genes quantified using quantitative real-time PCR. Adipogenic responses to 1, 25-(OH)(2)D(3) were determined on 6, and 12 h, and days 1-10 after induction of adipogenesis by a hormonal cocktail with or without 1, 25-(OH)(2)D(3). In response to 1, 25-(OH)(2)D(3) (1, 10, and 100 nM), lipid accumulation and the expression of PPARγ, C/EBPα, FABP4 and SCD-1 were inhibited through day 10, and vitamin D receptor expression was inhibited in the early time points. The greatest inhibitory effect was upon expression of FABP4. Expression of SREBP-1c was only affected on day 2. The lowest concentrations of 1, 25-(OH)(2)D(3) tested did not affect adipocyte differentiation or adipogenic gene expression. The C/EBPα promoter activity response to 1, 25-(OH)(2)D(3) was also tested, with no effect detected. These results indicate that 1, 25-(OH)(2)D(3) inhibited adipogenesis via suppressing adipogenic-specific genes, and is invoked either during PPARγ activation or immediately up-stream thereof. Gene expression down-stream of PPARγ especially FABP4 was strongly inhibited, and we suggest that the role of 1, 25-(OH)(2)D(3) in regulating adipogenesis will be informed by further studies of adipogenic-specific gene promoter activity. |
format | Online Article Text |
id | pubmed-4451075 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44510752015-06-09 Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells Ji, Shuhan Doumit, Matthew E. Hill, Rodney A. PLoS One Research Article The functions of 1, 25-dihydroxyvitamin D (1, 25-(OH)(2)D(3)) in regulating adipogenesis, adipocyte differentiation and key adipogenic gene expression were studied in 3T3-L1 preadipocytes. Five concentrations (0.01, 0.1, 1, 10, 100nM) of 1, 25-(OH)(2)D(3) were studied and lipid accumulation measured by Oil Red O staining and expression of adipogenic genes quantified using quantitative real-time PCR. Adipogenic responses to 1, 25-(OH)(2)D(3) were determined on 6, and 12 h, and days 1-10 after induction of adipogenesis by a hormonal cocktail with or without 1, 25-(OH)(2)D(3). In response to 1, 25-(OH)(2)D(3) (1, 10, and 100 nM), lipid accumulation and the expression of PPARγ, C/EBPα, FABP4 and SCD-1 were inhibited through day 10, and vitamin D receptor expression was inhibited in the early time points. The greatest inhibitory effect was upon expression of FABP4. Expression of SREBP-1c was only affected on day 2. The lowest concentrations of 1, 25-(OH)(2)D(3) tested did not affect adipocyte differentiation or adipogenic gene expression. The C/EBPα promoter activity response to 1, 25-(OH)(2)D(3) was also tested, with no effect detected. These results indicate that 1, 25-(OH)(2)D(3) inhibited adipogenesis via suppressing adipogenic-specific genes, and is invoked either during PPARγ activation or immediately up-stream thereof. Gene expression down-stream of PPARγ especially FABP4 was strongly inhibited, and we suggest that the role of 1, 25-(OH)(2)D(3) in regulating adipogenesis will be informed by further studies of adipogenic-specific gene promoter activity. Public Library of Science 2015-06-01 /pmc/articles/PMC4451075/ /pubmed/26030589 http://dx.doi.org/10.1371/journal.pone.0126142 Text en © 2015 Ji et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ji, Shuhan Doumit, Matthew E. Hill, Rodney A. Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells |
title | Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells |
title_full | Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells |
title_fullStr | Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells |
title_full_unstemmed | Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells |
title_short | Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells |
title_sort | regulation of adipogenesis and key adipogenic gene expression by 1, 25-dihydroxyvitamin d in 3t3-l1 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451075/ https://www.ncbi.nlm.nih.gov/pubmed/26030589 http://dx.doi.org/10.1371/journal.pone.0126142 |
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