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Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals

BACKGROUND: The bacterial genus Staphylococcus consists of many species that causes infections in pet animals. Antimicrobial resistant staphylococci cause infections that are difficult to treat and they are important from the point of one health perspective. The aim of this study was to determine th...

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Autores principales: Ruzauskas, Modestas, Couto, Natacha, Kerziene, Sigita, Siugzdiniene, Rita, Klimiene, Irena, Virgailis, Marius, Pomba, Constança
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451720/
https://www.ncbi.nlm.nih.gov/pubmed/26032539
http://dx.doi.org/10.1186/s13028-015-0117-z
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author Ruzauskas, Modestas
Couto, Natacha
Kerziene, Sigita
Siugzdiniene, Rita
Klimiene, Irena
Virgailis, Marius
Pomba, Constança
author_facet Ruzauskas, Modestas
Couto, Natacha
Kerziene, Sigita
Siugzdiniene, Rita
Klimiene, Irena
Virgailis, Marius
Pomba, Constança
author_sort Ruzauskas, Modestas
collection PubMed
description BACKGROUND: The bacterial genus Staphylococcus consists of many species that causes infections in pet animals. Antimicrobial resistant staphylococci cause infections that are difficult to treat and they are important from the point of one health perspective. The aim of this study was to determine the prevalence of methicillin-resistant Staphylococcus (MRS) species, including methicillin-resistant S. aureus (MRSA) in diseased pet animals (Group A) and kennel dogs (Group B) in Lithuania and to characterize the isolates according to their antimicrobial resistance. RESULTS: Twenty-one MRS isolates were obtained from 395 clinical samples (5.3 %; CI 95 % 3.5-8.0) of Group A animals. Sixteen, four and one isolates were from dogs, cats and a pet rabbit, respectively. The mecA gene was present in 20 isolates, whereas one isolate was positive for the mecC gene. Twenty-one MRS isolates (20.0 %; CI 95 % 13.5-28.6) were obtained from the vagina of female dogs (n = 105) (Group B). All isolates carried the mecA gene. Twelve MRS species were isolated of which S. pseudintermedius was the most common (18/42) followed by S. haemolyticus (8/42) and S. lentus (4/42). MRSA was not found. All MRS strains were susceptible to vancomycin, linezolid, daptomycin and quinupristin/dalfopristin. Resistance to tetracycline (16/21), clindamycin (15/21) and erythromycin (14/21) was the most common types of resistance in Group A animals. Three isolates also demonstrated resistance to rifampin. Resistance toward gentamicin (16/21), ciprofloxacin (15/21), macrolides (15/21) and tetracycline (12/21) was the most common in kennel dogs (Group B). The most common genes encoding resistance to antimicrobials (excluding beta-lactams) in isolates from Group A pets were tetK (21/42), aph(3′)-IIIa (11/42) and aac(6')-Ie-aph(2'')-Ia (9/42). CONCLUSIONS: A wide range of MRS species were found in pet animals in Lithuania. MRSA was not found.
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spelling pubmed-44517202015-06-03 Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals Ruzauskas, Modestas Couto, Natacha Kerziene, Sigita Siugzdiniene, Rita Klimiene, Irena Virgailis, Marius Pomba, Constança Acta Vet Scand Research BACKGROUND: The bacterial genus Staphylococcus consists of many species that causes infections in pet animals. Antimicrobial resistant staphylococci cause infections that are difficult to treat and they are important from the point of one health perspective. The aim of this study was to determine the prevalence of methicillin-resistant Staphylococcus (MRS) species, including methicillin-resistant S. aureus (MRSA) in diseased pet animals (Group A) and kennel dogs (Group B) in Lithuania and to characterize the isolates according to their antimicrobial resistance. RESULTS: Twenty-one MRS isolates were obtained from 395 clinical samples (5.3 %; CI 95 % 3.5-8.0) of Group A animals. Sixteen, four and one isolates were from dogs, cats and a pet rabbit, respectively. The mecA gene was present in 20 isolates, whereas one isolate was positive for the mecC gene. Twenty-one MRS isolates (20.0 %; CI 95 % 13.5-28.6) were obtained from the vagina of female dogs (n = 105) (Group B). All isolates carried the mecA gene. Twelve MRS species were isolated of which S. pseudintermedius was the most common (18/42) followed by S. haemolyticus (8/42) and S. lentus (4/42). MRSA was not found. All MRS strains were susceptible to vancomycin, linezolid, daptomycin and quinupristin/dalfopristin. Resistance to tetracycline (16/21), clindamycin (15/21) and erythromycin (14/21) was the most common types of resistance in Group A animals. Three isolates also demonstrated resistance to rifampin. Resistance toward gentamicin (16/21), ciprofloxacin (15/21), macrolides (15/21) and tetracycline (12/21) was the most common in kennel dogs (Group B). The most common genes encoding resistance to antimicrobials (excluding beta-lactams) in isolates from Group A pets were tetK (21/42), aph(3′)-IIIa (11/42) and aac(6')-Ie-aph(2'')-Ia (9/42). CONCLUSIONS: A wide range of MRS species were found in pet animals in Lithuania. MRSA was not found. BioMed Central 2015-06-02 /pmc/articles/PMC4451720/ /pubmed/26032539 http://dx.doi.org/10.1186/s13028-015-0117-z Text en © Ruzauskas et al. 2015
spellingShingle Research
Ruzauskas, Modestas
Couto, Natacha
Kerziene, Sigita
Siugzdiniene, Rita
Klimiene, Irena
Virgailis, Marius
Pomba, Constança
Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals
title Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals
title_full Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals
title_fullStr Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals
title_full_unstemmed Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals
title_short Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals
title_sort prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in lithuanian pet animals
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451720/
https://www.ncbi.nlm.nih.gov/pubmed/26032539
http://dx.doi.org/10.1186/s13028-015-0117-z
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