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Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia

BACKGROUND: Allopatric populations present challenges for biologists working with vectors. We suggest that conspecificity can be concluded in these cases when data from four character sets—chromosomal, ecological, molecular, and morphological—express variation no greater between the allopatric popul...

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Autores principales: Low, Van Lun, Adler, Peter H., Sofian-Azirun, Mohd, Srisuka, Wichai, Saeung, Atiporn, Huang, Yao-Te, Hadi, Upik Kesumawati, Da Pham, Xuan, Takaoka, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451747/
https://www.ncbi.nlm.nih.gov/pubmed/26022092
http://dx.doi.org/10.1186/s13071-015-0911-5
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author Low, Van Lun
Adler, Peter H.
Sofian-Azirun, Mohd
Srisuka, Wichai
Saeung, Atiporn
Huang, Yao-Te
Hadi, Upik Kesumawati
Da Pham, Xuan
Takaoka, Hiroyuki
author_facet Low, Van Lun
Adler, Peter H.
Sofian-Azirun, Mohd
Srisuka, Wichai
Saeung, Atiporn
Huang, Yao-Te
Hadi, Upik Kesumawati
Da Pham, Xuan
Takaoka, Hiroyuki
author_sort Low, Van Lun
collection PubMed
description BACKGROUND: Allopatric populations present challenges for biologists working with vectors. We suggest that conspecificity can be concluded in these cases when data from four character sets—chromosomal, ecological, molecular, and morphological—express variation no greater between the allopatric populations than between corresponding sympatric populations. We use this approach to test the conspecificity of Simulium nodosum Puri on the mainland of Southeast Asia and Simulium shirakii Kono & Takahasi in Taiwan. The validity of these two putative species has long been disputed given that they are morphologically indistinguishable. FINDINGS: The mitochondria-encoded cytochrome c oxidase subunit I (COI), 12S rRNA, and 16S rRNA genes and the nuclear-encoded 28S rRNA gene support the conspecific status of S. nodosum from Myanmar, Thailand, and Vietnam and S. shirakii from Taiwan; 0 to 0.19 % genetic differences between the two taxa suggest intraspecific polymorphism. The banding patterns of the polytene chromosomes of the insular Taiwanese population of S. shirakii and mainland populations of S. nodosum are congruent. The overlapping ranges of habitat characteristics and hosts of S. nodosum and S. shirakii corroborate the chromosomal, molecular, and morphological data. CONCLUSIONS: Four independent sources of evidence (chromosomes, DNA, ecology, and morphology) support the conspecificity of S. nodosum and S. shirakii. We, therefore, synonymize S. shirakii with S. nodosum. This study provides a guide for applying the procedure of testing conspecificity to other sets of allopatric vectors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-0911-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-44517472015-06-03 Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia Low, Van Lun Adler, Peter H. Sofian-Azirun, Mohd Srisuka, Wichai Saeung, Atiporn Huang, Yao-Te Hadi, Upik Kesumawati Da Pham, Xuan Takaoka, Hiroyuki Parasit Vectors Short Report BACKGROUND: Allopatric populations present challenges for biologists working with vectors. We suggest that conspecificity can be concluded in these cases when data from four character sets—chromosomal, ecological, molecular, and morphological—express variation no greater between the allopatric populations than between corresponding sympatric populations. We use this approach to test the conspecificity of Simulium nodosum Puri on the mainland of Southeast Asia and Simulium shirakii Kono & Takahasi in Taiwan. The validity of these two putative species has long been disputed given that they are morphologically indistinguishable. FINDINGS: The mitochondria-encoded cytochrome c oxidase subunit I (COI), 12S rRNA, and 16S rRNA genes and the nuclear-encoded 28S rRNA gene support the conspecific status of S. nodosum from Myanmar, Thailand, and Vietnam and S. shirakii from Taiwan; 0 to 0.19 % genetic differences between the two taxa suggest intraspecific polymorphism. The banding patterns of the polytene chromosomes of the insular Taiwanese population of S. shirakii and mainland populations of S. nodosum are congruent. The overlapping ranges of habitat characteristics and hosts of S. nodosum and S. shirakii corroborate the chromosomal, molecular, and morphological data. CONCLUSIONS: Four independent sources of evidence (chromosomes, DNA, ecology, and morphology) support the conspecificity of S. nodosum and S. shirakii. We, therefore, synonymize S. shirakii with S. nodosum. This study provides a guide for applying the procedure of testing conspecificity to other sets of allopatric vectors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-0911-5) contains supplementary material, which is available to authorized users. BioMed Central 2015-05-29 /pmc/articles/PMC4451747/ /pubmed/26022092 http://dx.doi.org/10.1186/s13071-015-0911-5 Text en © Low et al. 2015
spellingShingle Short Report
Low, Van Lun
Adler, Peter H.
Sofian-Azirun, Mohd
Srisuka, Wichai
Saeung, Atiporn
Huang, Yao-Te
Hadi, Upik Kesumawati
Da Pham, Xuan
Takaoka, Hiroyuki
Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia
title Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia
title_full Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia
title_fullStr Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia
title_full_unstemmed Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia
title_short Tests of conspecificity for allopatric vectors: Simulium nodosum and Simulium shirakii (Diptera: Simuliidae) in Asia
title_sort tests of conspecificity for allopatric vectors: simulium nodosum and simulium shirakii (diptera: simuliidae) in asia
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451747/
https://www.ncbi.nlm.nih.gov/pubmed/26022092
http://dx.doi.org/10.1186/s13071-015-0911-5
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