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Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis

Dispersion or dilution of stool by water from heavy rainfall may affect Strongyloides stercoralis free-living development producing infective filariform larvae (FL). This study examined effect of water dilution of stool on survival of S. stercoralis free-living development. One g of stool was prepar...

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Autores principales: Anamnart, Witthaya, Maleewong Intapan, Pewpan, Pattanawongsa, Attarat, Chamavit, Pennapa, Kaewsawat, Supreecha, Maleewong, Wanchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451808/
https://www.ncbi.nlm.nih.gov/pubmed/26035061
http://dx.doi.org/10.1038/srep10749
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author Anamnart, Witthaya
Maleewong Intapan, Pewpan
Pattanawongsa, Attarat
Chamavit, Pennapa
Kaewsawat, Supreecha
Maleewong, Wanchai
author_facet Anamnart, Witthaya
Maleewong Intapan, Pewpan
Pattanawongsa, Attarat
Chamavit, Pennapa
Kaewsawat, Supreecha
Maleewong, Wanchai
author_sort Anamnart, Witthaya
collection PubMed
description Dispersion or dilution of stool by water from heavy rainfall may affect Strongyloides stercoralis free-living development producing infective filariform larvae (FL). This study examined effect of water dilution of stool on survival of S. stercoralis free-living development. One g of stool was prepared in water so that its soluble component was diluted sequentially from 1:2 to 1:480. Three dishes were used to compare FL production in three culture conditions: stool suspension, stool sediment deposited in soil, and isolated rhabditiform larvae (RhL) deposited in soil. The fourth dish was for developmental observation of RhL into free-living stages. Numerous FL were generated from undiluted or 1:2 diluted stool and stool sediment placed on soil. However, starting from dilution 1:5, FL production continuously decreased in both stool suspensions and stool sediments placed on soil. RhL isolated from stool dilutions placed on soil gave rise to few FL. Worm mating were seen at 24-30 hours in dilutions 1:20-1:120 only. Highest numbers of FL from indirect free-living cycle were 1/3 of those from control. FL production decreased as stool dilution increased, and reached zero production at 1:160 dilution. Rainfall may disperse or dilute stool so that nutritional supplement for S. stercoralis free-living development is insufficient.
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spelling pubmed-44518082015-06-09 Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis Anamnart, Witthaya Maleewong Intapan, Pewpan Pattanawongsa, Attarat Chamavit, Pennapa Kaewsawat, Supreecha Maleewong, Wanchai Sci Rep Article Dispersion or dilution of stool by water from heavy rainfall may affect Strongyloides stercoralis free-living development producing infective filariform larvae (FL). This study examined effect of water dilution of stool on survival of S. stercoralis free-living development. One g of stool was prepared in water so that its soluble component was diluted sequentially from 1:2 to 1:480. Three dishes were used to compare FL production in three culture conditions: stool suspension, stool sediment deposited in soil, and isolated rhabditiform larvae (RhL) deposited in soil. The fourth dish was for developmental observation of RhL into free-living stages. Numerous FL were generated from undiluted or 1:2 diluted stool and stool sediment placed on soil. However, starting from dilution 1:5, FL production continuously decreased in both stool suspensions and stool sediments placed on soil. RhL isolated from stool dilutions placed on soil gave rise to few FL. Worm mating were seen at 24-30 hours in dilutions 1:20-1:120 only. Highest numbers of FL from indirect free-living cycle were 1/3 of those from control. FL production decreased as stool dilution increased, and reached zero production at 1:160 dilution. Rainfall may disperse or dilute stool so that nutritional supplement for S. stercoralis free-living development is insufficient. Nature Publishing Group 2015-06-02 /pmc/articles/PMC4451808/ /pubmed/26035061 http://dx.doi.org/10.1038/srep10749 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Anamnart, Witthaya
Maleewong Intapan, Pewpan
Pattanawongsa, Attarat
Chamavit, Pennapa
Kaewsawat, Supreecha
Maleewong, Wanchai
Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis
title Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis
title_full Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis
title_fullStr Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis
title_full_unstemmed Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis
title_short Effect of dilution of stool soluble component on growth and development of Strongyloides stercoralis
title_sort effect of dilution of stool soluble component on growth and development of strongyloides stercoralis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451808/
https://www.ncbi.nlm.nih.gov/pubmed/26035061
http://dx.doi.org/10.1038/srep10749
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