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Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B
Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson's disease (PD) model via a 6-OHDA intrastriatal inj...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451977/ https://www.ncbi.nlm.nih.gov/pubmed/26090233 http://dx.doi.org/10.1155/2015/387028 |
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author | Batassini, Cristiane Broetto, Núbia Tortorelli, Lucas Silva Borsoi, Milene Zanotto, Caroline Galland, Fabiana Souza, Tadeu Mello Leite, Marina Concli Gonçalves, Carlos-Alberto |
author_facet | Batassini, Cristiane Broetto, Núbia Tortorelli, Lucas Silva Borsoi, Milene Zanotto, Caroline Galland, Fabiana Souza, Tadeu Mello Leite, Marina Concli Gonçalves, Carlos-Alberto |
author_sort | Batassini, Cristiane |
collection | PubMed |
description | Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson's disease (PD) model via a 6-OHDA intrastriatal injection in rats and investigated the changes in GFAP and S100B using ELISA in the substantia nigra (SN), striatum, and cerebrospinal fluid on the 1st, 7th, and 21st days following the injection. The model was validated using measurements of rotational behaviour induced by methylphenidate and tyrosine hydroxylase in the dopaminergic pathway. To our knowledge, this is the first measurement of cerebrospinal fluid S100B and GFAP in the 6-OHDA model of PD. Gliosis (based on a GFAP increase) was identified in the striatum, but not in the SN. We identified a transitory increment of cerebrospinal fluid S100B and GFAP on the 1st and 7th days, respectively. This initial change in cerebrospinal fluid S100B was apparently related to the mechanical lesion. However, the 6-OHDA-induced S100B secretion was confirmed in astrocyte cultures. Current data reinforce the idea that glial changes precede neuronal damage in PD; however, these findings also indicate that caution is necessary regarding the interpretation of data in this PD model. |
format | Online Article Text |
id | pubmed-4451977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44519772015-06-18 Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B Batassini, Cristiane Broetto, Núbia Tortorelli, Lucas Silva Borsoi, Milene Zanotto, Caroline Galland, Fabiana Souza, Tadeu Mello Leite, Marina Concli Gonçalves, Carlos-Alberto Neural Plast Research Article Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson's disease (PD) model via a 6-OHDA intrastriatal injection in rats and investigated the changes in GFAP and S100B using ELISA in the substantia nigra (SN), striatum, and cerebrospinal fluid on the 1st, 7th, and 21st days following the injection. The model was validated using measurements of rotational behaviour induced by methylphenidate and tyrosine hydroxylase in the dopaminergic pathway. To our knowledge, this is the first measurement of cerebrospinal fluid S100B and GFAP in the 6-OHDA model of PD. Gliosis (based on a GFAP increase) was identified in the striatum, but not in the SN. We identified a transitory increment of cerebrospinal fluid S100B and GFAP on the 1st and 7th days, respectively. This initial change in cerebrospinal fluid S100B was apparently related to the mechanical lesion. However, the 6-OHDA-induced S100B secretion was confirmed in astrocyte cultures. Current data reinforce the idea that glial changes precede neuronal damage in PD; however, these findings also indicate that caution is necessary regarding the interpretation of data in this PD model. Hindawi Publishing Corporation 2015 2015-05-18 /pmc/articles/PMC4451977/ /pubmed/26090233 http://dx.doi.org/10.1155/2015/387028 Text en Copyright © 2015 Cristiane Batassini et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Batassini, Cristiane Broetto, Núbia Tortorelli, Lucas Silva Borsoi, Milene Zanotto, Caroline Galland, Fabiana Souza, Tadeu Mello Leite, Marina Concli Gonçalves, Carlos-Alberto Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
title | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
title_full | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
title_fullStr | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
title_full_unstemmed | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
title_short | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
title_sort | striatal injury with 6-ohda transiently increases cerebrospinal gfap and s100b |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451977/ https://www.ncbi.nlm.nih.gov/pubmed/26090233 http://dx.doi.org/10.1155/2015/387028 |
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