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Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro

BACKGROUND: In the previous studies, telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to CD34 and c-kit. Therefore, the present study aimed to explore if telocytes could produce angiogenesis associated cytokines, promote...

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Autores principales: Zheng, Yonghua, Chen, Xiaoke, Qian, Mengjia, Zhang, Miaomiao, Zhang, Ding, Bai, Chunxue, Wang, Qun, Wang, Xiangdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452074/
https://www.ncbi.nlm.nih.gov/pubmed/26056572
http://dx.doi.org/10.1186/2052-8426-2-3
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author Zheng, Yonghua
Chen, Xiaoke
Qian, Mengjia
Zhang, Miaomiao
Zhang, Ding
Bai, Chunxue
Wang, Qun
Wang, Xiangdong
author_facet Zheng, Yonghua
Chen, Xiaoke
Qian, Mengjia
Zhang, Miaomiao
Zhang, Ding
Bai, Chunxue
Wang, Qun
Wang, Xiangdong
author_sort Zheng, Yonghua
collection PubMed
description BACKGROUND: In the previous studies, telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to CD34 and c-kit. Therefore, the present study aimed to explore if telocytes could produce angiogenesis associated cytokines, promote the proliferation and the angiogenesis of vascular endothelial cells in vitro. METHODS: Human lung telocytes were isolated and cultured, and were identified by immunofluorescence cytochemistry with CD34, c-kit and vimentin. Telocytes conditional media (TCM) was prepared, and the expressions of angiogenesis associated cytokines in TCM were detected by ELISA. Human pulmonary microvascular endothelial cells (HPMECs) were cultured with DMEM media or TCM for 72 hours. The proliferation of HPMECs was continuously detected with CCK-8 kit at an interval of 12 hours. HPMECs were also injured by lipopolysaccharide, and cultured with TCM and DMEM respectively, and the tube formation capacity was detected. RESULTS: Telocytes were positive for CD34, c-kit and vimentin. The expressions of VEGF and EGF in TCM were significantly higher, the proliferation of HPMECs cultured with TCM significantly increased, and the tube formation of HPMECs injured by endotoxin was improved with the culture of TCM, as compared with the culture of DMEM. CONCLUSION: The present study provides the evidence that human lung telocytes could produce the growth factors, such as VEGF and EGF. Telocytes conditional media induced the proliferation of pulmonary endothelial cells and prevented from endotoxin-induced compromise of pulmonary endothelial angiogenesis.
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spelling pubmed-44520742015-06-09 Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro Zheng, Yonghua Chen, Xiaoke Qian, Mengjia Zhang, Miaomiao Zhang, Ding Bai, Chunxue Wang, Qun Wang, Xiangdong Mol Cell Ther Research Article BACKGROUND: In the previous studies, telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to CD34 and c-kit. Therefore, the present study aimed to explore if telocytes could produce angiogenesis associated cytokines, promote the proliferation and the angiogenesis of vascular endothelial cells in vitro. METHODS: Human lung telocytes were isolated and cultured, and were identified by immunofluorescence cytochemistry with CD34, c-kit and vimentin. Telocytes conditional media (TCM) was prepared, and the expressions of angiogenesis associated cytokines in TCM were detected by ELISA. Human pulmonary microvascular endothelial cells (HPMECs) were cultured with DMEM media or TCM for 72 hours. The proliferation of HPMECs was continuously detected with CCK-8 kit at an interval of 12 hours. HPMECs were also injured by lipopolysaccharide, and cultured with TCM and DMEM respectively, and the tube formation capacity was detected. RESULTS: Telocytes were positive for CD34, c-kit and vimentin. The expressions of VEGF and EGF in TCM were significantly higher, the proliferation of HPMECs cultured with TCM significantly increased, and the tube formation of HPMECs injured by endotoxin was improved with the culture of TCM, as compared with the culture of DMEM. CONCLUSION: The present study provides the evidence that human lung telocytes could produce the growth factors, such as VEGF and EGF. Telocytes conditional media induced the proliferation of pulmonary endothelial cells and prevented from endotoxin-induced compromise of pulmonary endothelial angiogenesis. BioMed Central 2014-02-01 /pmc/articles/PMC4452074/ /pubmed/26056572 http://dx.doi.org/10.1186/2052-8426-2-3 Text en © Zheng et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zheng, Yonghua
Chen, Xiaoke
Qian, Mengjia
Zhang, Miaomiao
Zhang, Ding
Bai, Chunxue
Wang, Qun
Wang, Xiangdong
Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
title Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
title_full Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
title_fullStr Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
title_full_unstemmed Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
title_short Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
title_sort human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452074/
https://www.ncbi.nlm.nih.gov/pubmed/26056572
http://dx.doi.org/10.1186/2052-8426-2-3
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