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A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats

BACKGROUND: Gut microflora play a crucial role in the biotransformation of ginsenosides to compound K (CK), which may affect the pharmacological effects of ginseng. Prebiotics, such as NUTRIOSE, could enhance the formation and consequent absorption of CK through the modulation of gut microbial metab...

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Autores principales: Kim, Kyung-Ah, Yoo, Hye Hyun, Gu, Wan, Yu, Dae-Hyung, Jin, Ming Ji, Choi, Hae-Lim, Yuan, Kathy, Guerin-Deremaux, Laetitia, Kim, Dong-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452535/
https://www.ncbi.nlm.nih.gov/pubmed/26045693
http://dx.doi.org/10.1016/j.jgr.2014.11.002
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author Kim, Kyung-Ah
Yoo, Hye Hyun
Gu, Wan
Yu, Dae-Hyung
Jin, Ming Ji
Choi, Hae-Lim
Yuan, Kathy
Guerin-Deremaux, Laetitia
Kim, Dong-Hyun
author_facet Kim, Kyung-Ah
Yoo, Hye Hyun
Gu, Wan
Yu, Dae-Hyung
Jin, Ming Ji
Choi, Hae-Lim
Yuan, Kathy
Guerin-Deremaux, Laetitia
Kim, Dong-Hyun
author_sort Kim, Kyung-Ah
collection PubMed
description BACKGROUND: Gut microflora play a crucial role in the biotransformation of ginsenosides to compound K (CK), which may affect the pharmacological effects of ginseng. Prebiotics, such as NUTRIOSE, could enhance the formation and consequent absorption of CK through the modulation of gut microbial metabolic activities. In this study, the effect of a prebiotic fiber (NUTRIOSE) on the pharmacokinetics of ginsenoside CK, a bioactive metabolite of ginsenosides, and its mechanism of action were investigated. METHODS: Male Sprague–Dawley rats were given control or NUTRIOSE-containing diets (control diet + NUTRIOSE) for 2 wk, and ginseng extract or vehicle was then orally administered. Blood samples were collected to investigate the pharmacokinetics of CK using liquid chromatography–tandem mass spectrometry. Fecal activities that metabolize ginsenoside Rb1 to CK were assayed with fecal specimens or bacteria cultures. RESULTS: When ginseng extract was orally administered to rats fed with 2.5%, 5%, or 10% NUTRIOSE containing diets, the maximum plasma concentration (C(max)) and area under the plasma concentration–time curve values of CK significantly increased in a NUTRIOSE content-dependent manner. NUTRIOSE intake increased glycosidase activity and CK formation in rat intestinal contents. The CK-forming activities of intestinal microbiota cultured in vitro were significantly induced by NUTRIOSE. CONCLUSION: These results show that prebiotic diets, such as NUTRIOSE, may promote the metabolic conversion of ginsenosides to CK and the subsequent absorption of CK in the gastrointestinal tract and may potentiate the pharmacological effects of ginseng.
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spelling pubmed-44525352015-06-04 A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats Kim, Kyung-Ah Yoo, Hye Hyun Gu, Wan Yu, Dae-Hyung Jin, Ming Ji Choi, Hae-Lim Yuan, Kathy Guerin-Deremaux, Laetitia Kim, Dong-Hyun J Ginseng Res Research Article BACKGROUND: Gut microflora play a crucial role in the biotransformation of ginsenosides to compound K (CK), which may affect the pharmacological effects of ginseng. Prebiotics, such as NUTRIOSE, could enhance the formation and consequent absorption of CK through the modulation of gut microbial metabolic activities. In this study, the effect of a prebiotic fiber (NUTRIOSE) on the pharmacokinetics of ginsenoside CK, a bioactive metabolite of ginsenosides, and its mechanism of action were investigated. METHODS: Male Sprague–Dawley rats were given control or NUTRIOSE-containing diets (control diet + NUTRIOSE) for 2 wk, and ginseng extract or vehicle was then orally administered. Blood samples were collected to investigate the pharmacokinetics of CK using liquid chromatography–tandem mass spectrometry. Fecal activities that metabolize ginsenoside Rb1 to CK were assayed with fecal specimens or bacteria cultures. RESULTS: When ginseng extract was orally administered to rats fed with 2.5%, 5%, or 10% NUTRIOSE containing diets, the maximum plasma concentration (C(max)) and area under the plasma concentration–time curve values of CK significantly increased in a NUTRIOSE content-dependent manner. NUTRIOSE intake increased glycosidase activity and CK formation in rat intestinal contents. The CK-forming activities of intestinal microbiota cultured in vitro were significantly induced by NUTRIOSE. CONCLUSION: These results show that prebiotic diets, such as NUTRIOSE, may promote the metabolic conversion of ginsenosides to CK and the subsequent absorption of CK in the gastrointestinal tract and may potentiate the pharmacological effects of ginseng. Elsevier 2015-04 2014-11-24 /pmc/articles/PMC4452535/ /pubmed/26045693 http://dx.doi.org/10.1016/j.jgr.2014.11.002 Text en Copyright © 2014, The Korean Society of Ginseng, Published by Elsevier. All rights reserved. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Research Article
Kim, Kyung-Ah
Yoo, Hye Hyun
Gu, Wan
Yu, Dae-Hyung
Jin, Ming Ji
Choi, Hae-Lim
Yuan, Kathy
Guerin-Deremaux, Laetitia
Kim, Dong-Hyun
A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats
title A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats
title_full A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats
title_fullStr A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats
title_full_unstemmed A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats
title_short A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats
title_sort prebiotic fiber increases the formation and subsequent absorption of compound k following oral administration of ginseng in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452535/
https://www.ncbi.nlm.nih.gov/pubmed/26045693
http://dx.doi.org/10.1016/j.jgr.2014.11.002
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