Cargando…
Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes
The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infe...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452545/ https://www.ncbi.nlm.nih.gov/pubmed/26030198 http://dx.doi.org/10.1371/journal.pone.0127710 |
_version_ | 1782374318351581184 |
---|---|
author | Amigo, Natalia Mercado, Elsa Bentancor, Adriana Singh, Pallavi Vilte, Daniel Gerhardt, Elisabeth Zotta, Elsa Ibarra, Cristina Manning, Shannon D. Larzábal, Mariano Cataldi, Angel |
author_facet | Amigo, Natalia Mercado, Elsa Bentancor, Adriana Singh, Pallavi Vilte, Daniel Gerhardt, Elisabeth Zotta, Elsa Ibarra, Cristina Manning, Shannon D. Larzábal, Mariano Cataldi, Angel |
author_sort | Amigo, Natalia |
collection | PubMed |
description | The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina. |
format | Online Article Text |
id | pubmed-4452545 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44525452015-06-09 Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes Amigo, Natalia Mercado, Elsa Bentancor, Adriana Singh, Pallavi Vilte, Daniel Gerhardt, Elisabeth Zotta, Elsa Ibarra, Cristina Manning, Shannon D. Larzábal, Mariano Cataldi, Angel PLoS One Research Article The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina. Public Library of Science 2015-06-01 /pmc/articles/PMC4452545/ /pubmed/26030198 http://dx.doi.org/10.1371/journal.pone.0127710 Text en © 2015 Amigo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Amigo, Natalia Mercado, Elsa Bentancor, Adriana Singh, Pallavi Vilte, Daniel Gerhardt, Elisabeth Zotta, Elsa Ibarra, Cristina Manning, Shannon D. Larzábal, Mariano Cataldi, Angel Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes |
title | Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes |
title_full | Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes |
title_fullStr | Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes |
title_full_unstemmed | Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes |
title_short | Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes |
title_sort | clade 8 and clade 6 strains of escherichia coli o157:h7 from cattle in argentina have hypervirulent-like phenotypes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452545/ https://www.ncbi.nlm.nih.gov/pubmed/26030198 http://dx.doi.org/10.1371/journal.pone.0127710 |
work_keys_str_mv | AT amigonatalia clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT mercadoelsa clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT bentancoradriana clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT singhpallavi clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT viltedaniel clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT gerhardtelisabeth clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT zottaelsa clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT ibarracristina clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT manningshannond clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT larzabalmariano clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes AT cataldiangel clade8andclade6strainsofescherichiacolio157h7fromcattleinargentinahavehypervirulentlikephenotypes |