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HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland
This study was performed to assess attribution of high grade cervical intraepithelial neoplasia (HG-CIN) and invasive cervical cancer (ICC) to human papillomavirus (HPV) genotypes and secondarily to assess reproducibility of HG-CIN/ICC diagnosis obtained in Poland. Formaldehyde fixed, paraffin embed...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Netherlands
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452616/ https://www.ncbi.nlm.nih.gov/pubmed/25547828 http://dx.doi.org/10.1007/s12253-014-9877-4 |
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author | Nowakowski, Andrzej de Souza, Sabrina Collas Jach, Robert Rosillon, Dominique Książek, Alicja Holl, Katsiaryna |
author_facet | Nowakowski, Andrzej de Souza, Sabrina Collas Jach, Robert Rosillon, Dominique Książek, Alicja Holl, Katsiaryna |
author_sort | Nowakowski, Andrzej |
collection | PubMed |
description | This study was performed to assess attribution of high grade cervical intraepithelial neoplasia (HG-CIN) and invasive cervical cancer (ICC) to human papillomavirus (HPV) genotypes and secondarily to assess reproducibility of HG-CIN/ICC diagnosis obtained in Poland. Formaldehyde fixed, paraffin embedded blocks of HG-CIN/ICC from two distant institutions were sent to a central laboratory together with original histological diagnoses. Central/expert review of histopathological specimens was performed and agreement between local and central/expert diagnoses was calculated. HPV detection and genotyping in the samples was carried out with the use of SPF10-LiPA25 technology. Results were analyzed for 205 HG-CIN and 193 ICC cases with centrally confirmed diagnoses. Kappa coefficients and 95 % confidence intervals for HG-CIN and ICC diagnoses were: 0.13 (0.09;0.17) and 0.19 (0.11;0.26) respectively. Cohen’s kappa coefficients for lesions with representative number of samples ranged from 0.01 for cervical intraepithelial neoplasia grade 2 to 0.75 for adenocarcinoma. HPV DNA was detected in 96.1 and 91.2 % of the confirmed HG-CIN and ICC specimens respectively. HPV positive HG-CIN was most commonly attributed to HPV types: 16 (62.8), 33 (7.8), 31 (6.6), 52 (3.7), 45 (2.6) and 58 (2.6 %). HPV positive ICC was most commonly attributed to HPV types: 16 (72.1), 18 (10.8), 33 (5.7), 45 (3.4) and 31 (1.7 %). Reproducibility of histological diagnosis of HG-CIN/ICC obtained in Poland generally increases with the severity of lesion and is lowest for cervical intraepithelial neoplasia grade 2 and highest for adenocarcinoma. Over 80 % of ICC cases are vaccine-preventable in Poland. |
format | Online Article Text |
id | pubmed-4452616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-44526162015-06-05 HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland Nowakowski, Andrzej de Souza, Sabrina Collas Jach, Robert Rosillon, Dominique Książek, Alicja Holl, Katsiaryna Pathol Oncol Res Research This study was performed to assess attribution of high grade cervical intraepithelial neoplasia (HG-CIN) and invasive cervical cancer (ICC) to human papillomavirus (HPV) genotypes and secondarily to assess reproducibility of HG-CIN/ICC diagnosis obtained in Poland. Formaldehyde fixed, paraffin embedded blocks of HG-CIN/ICC from two distant institutions were sent to a central laboratory together with original histological diagnoses. Central/expert review of histopathological specimens was performed and agreement between local and central/expert diagnoses was calculated. HPV detection and genotyping in the samples was carried out with the use of SPF10-LiPA25 technology. Results were analyzed for 205 HG-CIN and 193 ICC cases with centrally confirmed diagnoses. Kappa coefficients and 95 % confidence intervals for HG-CIN and ICC diagnoses were: 0.13 (0.09;0.17) and 0.19 (0.11;0.26) respectively. Cohen’s kappa coefficients for lesions with representative number of samples ranged from 0.01 for cervical intraepithelial neoplasia grade 2 to 0.75 for adenocarcinoma. HPV DNA was detected in 96.1 and 91.2 % of the confirmed HG-CIN and ICC specimens respectively. HPV positive HG-CIN was most commonly attributed to HPV types: 16 (62.8), 33 (7.8), 31 (6.6), 52 (3.7), 45 (2.6) and 58 (2.6 %). HPV positive ICC was most commonly attributed to HPV types: 16 (72.1), 18 (10.8), 33 (5.7), 45 (3.4) and 31 (1.7 %). Reproducibility of histological diagnosis of HG-CIN/ICC obtained in Poland generally increases with the severity of lesion and is lowest for cervical intraepithelial neoplasia grade 2 and highest for adenocarcinoma. Over 80 % of ICC cases are vaccine-preventable in Poland. Springer Netherlands 2014-12-30 2015 /pmc/articles/PMC4452616/ /pubmed/25547828 http://dx.doi.org/10.1007/s12253-014-9877-4 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Research Nowakowski, Andrzej de Souza, Sabrina Collas Jach, Robert Rosillon, Dominique Książek, Alicja Holl, Katsiaryna HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland |
title | HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland |
title_full | HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland |
title_fullStr | HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland |
title_full_unstemmed | HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland |
title_short | HPV-Type Distribution and Reproducibility of Histological Diagnosis in Cervical Neoplasia in Poland |
title_sort | hpv-type distribution and reproducibility of histological diagnosis in cervical neoplasia in poland |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452616/ https://www.ncbi.nlm.nih.gov/pubmed/25547828 http://dx.doi.org/10.1007/s12253-014-9877-4 |
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