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Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach

Fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome of fomites from various sources. Paper currency notes are one of the most commonly exchanged objects and its potential to transmit pathogenic organisms has been well rec...

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Autores principales: Jalali, Saakshi, Kohli, Samantha, Latka, Chitra, Bhatia, Sugandha, Vellarikal, Shamsudheen Karuthedath, Sivasubbu, Sridhar, Scaria, Vinod, Ramachandran, Srinivasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452720/
https://www.ncbi.nlm.nih.gov/pubmed/26035208
http://dx.doi.org/10.1371/journal.pone.0128711
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author Jalali, Saakshi
Kohli, Samantha
Latka, Chitra
Bhatia, Sugandha
Vellarikal, Shamsudheen Karuthedath
Sivasubbu, Sridhar
Scaria, Vinod
Ramachandran, Srinivasan
author_facet Jalali, Saakshi
Kohli, Samantha
Latka, Chitra
Bhatia, Sugandha
Vellarikal, Shamsudheen Karuthedath
Sivasubbu, Sridhar
Scaria, Vinod
Ramachandran, Srinivasan
author_sort Jalali, Saakshi
collection PubMed
description Fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome of fomites from various sources. Paper currency notes are one of the most commonly exchanged objects and its potential to transmit pathogenic organisms has been well recognized. Approaches to identify the microbiome associated with paper currency notes have been largely limited to culture dependent approaches. Subsequent studies portrayed the use of 16S ribosomal RNA based approaches which provided insights into the taxonomical distribution of the microbiome. However, recent techniques including shotgun sequencing provides resolution at gene level and enable estimation of their copy numbers in the metagenome. We investigated the microbiome of Indian paper currency notes using a shotgun metagenome sequencing approach. Metagenomic DNA isolated from samples of frequently circulated denominations of Indian currency notes were sequenced using Illumina Hiseq sequencer. Analysis of the data revealed presence of species belonging to both eukaryotic and prokaryotic genera. The taxonomic distribution at kingdom level revealed contigs mapping to eukaryota (70%), bacteria (9%), viruses and archae (~1%). We identified 78 pathogens including Staphylococcus aureus, Corynebacterium glutamicum, Enterococcus faecalis, and 75 cellulose degrading organisms including Acidothermus cellulolyticus, Cellulomonas flavigena and Ruminococcus albus. Additionally, 78 antibiotic resistance genes were identified and 18 of these were found in all the samples. Furthermore, six out of 78 pathogens harbored at least one of the 18 common antibiotic resistance genes. To the best of our knowledge, this is the first report of shotgun metagenome sequence dataset of paper currency notes, which can be useful for future applications including as bio-surveillance of exchangeable fomites for infectious agents.
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spelling pubmed-44527202015-06-09 Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach Jalali, Saakshi Kohli, Samantha Latka, Chitra Bhatia, Sugandha Vellarikal, Shamsudheen Karuthedath Sivasubbu, Sridhar Scaria, Vinod Ramachandran, Srinivasan PLoS One Research Article Fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome of fomites from various sources. Paper currency notes are one of the most commonly exchanged objects and its potential to transmit pathogenic organisms has been well recognized. Approaches to identify the microbiome associated with paper currency notes have been largely limited to culture dependent approaches. Subsequent studies portrayed the use of 16S ribosomal RNA based approaches which provided insights into the taxonomical distribution of the microbiome. However, recent techniques including shotgun sequencing provides resolution at gene level and enable estimation of their copy numbers in the metagenome. We investigated the microbiome of Indian paper currency notes using a shotgun metagenome sequencing approach. Metagenomic DNA isolated from samples of frequently circulated denominations of Indian currency notes were sequenced using Illumina Hiseq sequencer. Analysis of the data revealed presence of species belonging to both eukaryotic and prokaryotic genera. The taxonomic distribution at kingdom level revealed contigs mapping to eukaryota (70%), bacteria (9%), viruses and archae (~1%). We identified 78 pathogens including Staphylococcus aureus, Corynebacterium glutamicum, Enterococcus faecalis, and 75 cellulose degrading organisms including Acidothermus cellulolyticus, Cellulomonas flavigena and Ruminococcus albus. Additionally, 78 antibiotic resistance genes were identified and 18 of these were found in all the samples. Furthermore, six out of 78 pathogens harbored at least one of the 18 common antibiotic resistance genes. To the best of our knowledge, this is the first report of shotgun metagenome sequence dataset of paper currency notes, which can be useful for future applications including as bio-surveillance of exchangeable fomites for infectious agents. Public Library of Science 2015-06-02 /pmc/articles/PMC4452720/ /pubmed/26035208 http://dx.doi.org/10.1371/journal.pone.0128711 Text en © 2015 Jalali et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jalali, Saakshi
Kohli, Samantha
Latka, Chitra
Bhatia, Sugandha
Vellarikal, Shamsudheen Karuthedath
Sivasubbu, Sridhar
Scaria, Vinod
Ramachandran, Srinivasan
Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach
title Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach
title_full Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach
title_fullStr Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach
title_full_unstemmed Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach
title_short Screening Currency Notes for Microbial Pathogens and Antibiotic Resistance Genes Using a Shotgun Metagenomic Approach
title_sort screening currency notes for microbial pathogens and antibiotic resistance genes using a shotgun metagenomic approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452720/
https://www.ncbi.nlm.nih.gov/pubmed/26035208
http://dx.doi.org/10.1371/journal.pone.0128711
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