Cargando…

The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents

Negative staining electron microscopy methods can be employed for the diagnosis of viral particles in animal samples. In fact, negative staining electron microscopy methods are used to identify viruses, especially in minor species and wild animals, when no other methods are available and in cases of...

Descripción completa

Detalles Bibliográficos
Autores principales: Lavazza, Antonio, Tittarelli, Cristiana, Cerioli, Monica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452926/
https://www.ncbi.nlm.nih.gov/pubmed/26008707
http://dx.doi.org/10.3390/v7052683
_version_ 1782374394225491968
author Lavazza, Antonio
Tittarelli, Cristiana
Cerioli, Monica
author_facet Lavazza, Antonio
Tittarelli, Cristiana
Cerioli, Monica
author_sort Lavazza, Antonio
collection PubMed
description Negative staining electron microscopy methods can be employed for the diagnosis of viral particles in animal samples. In fact, negative staining electron microscopy methods are used to identify viruses, especially in minor species and wild animals, when no other methods are available and in cases of rare, emerging or re-emerging infections. In particular, immune-electron-microscopy with convalescent sera is employed to detect etiological agents when there are undiagnosed clinical outbreaks, when alternative diagnostic methods fail due to the lack of immunological reagents and primers, and when there is no indicative clinical suspect. An overview of immune-electron-microscopy with convalescent sera’s use in the diagnosis of new and unsuspected viruses in animals of domestic and wild species is provided through the descriptions of the following four diagnostic veterinary cases: (I) enteric viruses of pigs: Porcine Rotavirus, Porcine Epidemic Diarrhea Virus, Porcine Circovirus and Porcine Torovirus; (II) Rotavirus and astrovirus in young turkeys with enteritis; (III) Parvovirus-like particles in pheasants; and (IV) Lagoviruses: Rabbit Hemorrhagic Disease Virus and European Brown Hare Syndrome Virus.
format Online
Article
Text
id pubmed-4452926
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-44529262015-06-04 The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents Lavazza, Antonio Tittarelli, Cristiana Cerioli, Monica Viruses Article Negative staining electron microscopy methods can be employed for the diagnosis of viral particles in animal samples. In fact, negative staining electron microscopy methods are used to identify viruses, especially in minor species and wild animals, when no other methods are available and in cases of rare, emerging or re-emerging infections. In particular, immune-electron-microscopy with convalescent sera is employed to detect etiological agents when there are undiagnosed clinical outbreaks, when alternative diagnostic methods fail due to the lack of immunological reagents and primers, and when there is no indicative clinical suspect. An overview of immune-electron-microscopy with convalescent sera’s use in the diagnosis of new and unsuspected viruses in animals of domestic and wild species is provided through the descriptions of the following four diagnostic veterinary cases: (I) enteric viruses of pigs: Porcine Rotavirus, Porcine Epidemic Diarrhea Virus, Porcine Circovirus and Porcine Torovirus; (II) Rotavirus and astrovirus in young turkeys with enteritis; (III) Parvovirus-like particles in pheasants; and (IV) Lagoviruses: Rabbit Hemorrhagic Disease Virus and European Brown Hare Syndrome Virus. MDPI 2015-05-22 /pmc/articles/PMC4452926/ /pubmed/26008707 http://dx.doi.org/10.3390/v7052683 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lavazza, Antonio
Tittarelli, Cristiana
Cerioli, Monica
The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents
title The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents
title_full The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents
title_fullStr The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents
title_full_unstemmed The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents
title_short The Use of Convalescent Sera in Immune-Electron Microscopy to Detect Non-Suspected/New Viral Agents
title_sort use of convalescent sera in immune-electron microscopy to detect non-suspected/new viral agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452926/
https://www.ncbi.nlm.nih.gov/pubmed/26008707
http://dx.doi.org/10.3390/v7052683
work_keys_str_mv AT lavazzaantonio theuseofconvalescentserainimmuneelectronmicroscopytodetectnonsuspectednewviralagents
AT tittarellicristiana theuseofconvalescentserainimmuneelectronmicroscopytodetectnonsuspectednewviralagents
AT ceriolimonica theuseofconvalescentserainimmuneelectronmicroscopytodetectnonsuspectednewviralagents
AT lavazzaantonio useofconvalescentserainimmuneelectronmicroscopytodetectnonsuspectednewviralagents
AT tittarellicristiana useofconvalescentserainimmuneelectronmicroscopytodetectnonsuspectednewviralagents
AT ceriolimonica useofconvalescentserainimmuneelectronmicroscopytodetectnonsuspectednewviralagents